ZytoLight Fluorescent in situ Hybridization (FISH) Probes and Kits

HER-2 neu ZytoLight FISH Probe on an FFPE Breast Carcinoma

EGFR ZytoLight FISH Probe on an FFPE Colon Carcinoma

MDM2 ZytoLight FISH Probes on an FFPE Liposcarcinoma

The ZytoLight system uses direct labeled FISH probes (1), eliminating the need to detect the probes with fluorophore-coupled antibodies. The probes are detected by fluorescence microscopy using appropriate filter sets (2). Due to an exciter filter (3), full-spectrum light, emitted by the microscope lamp (4), is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror (5). The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter (6) reduces the emitted light to a defined wavelength that can be detected.

ZytoLight FISH probes are directly labeled using the unique ZytoLight Direct Label System II providing improved signal intensity. All ZytoLight single copy (SPEC) probes are processed by the unique ZytoLight Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!

ZytoLight CEN probes hybridize to highly repetitive human satellite DNA sequences located at the centromeric regions of chromosomes producing sharp, bright signals specific for each individual chromosome.   

For making FISH analysis reliable and user-friendly, all ZytoLight FISH probes can be combined with the ZytoLight FISH-Tissue Implementation Kit (Z-2028-20) for FISH analyses on tissue specimens. Implementation Kits include all necessary pretreatment solutions, wash buffers and DAPI/Antifade solution and a detailed protocol to perform successful FISH experiments. Additionally, for some major targets, complete kits including probes and all necessary reagents are available.