HPV ZytoFast CISH Probe on an FFPE Cervical Cancer
EBV ZytoFast CISH Probe on an FFPE Hodgkin’s Lymphoma
Ig-Kappa/Lambda ZytoFast CISH Probe on an FFPE Tonsil Tissue
Achieving Chromogenic in situ Hybridization Results in Just 4 Hours!
Introduction
The ZytoFast® products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-κ and Ig-λ light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples. The Signal intensity of ZytoFast probes is increased even more when using the ZytoFast PLUS Implementation Kits
Method Description – ZytoFast
The ZytoFast system uses oligonucleotide probes tagged with Biotin (1) which are detected using enzyme-conjugated streptavidin targeting the tag (2).
The enzymatic reaction of chromogenic substrates (3), e.g. BCIP/NBT or AEC, leads to the formation of strong color precipitates that can be visualized by light microscopy.
Method Description – ZytoFast PLUS
The ZytoFast PLUS system uses Digoxigenin-labeled probes (1) which are detected using primary antibodies (2). These antibodies are detected by polymerized enzyme-conjugated secondary antibodies (3). The enzymatic reaction of chromogenic substrates (4), e.g. NBT/BCIP, AEC or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.
High Sensitivity and Specificty
All ZytoFast probes are tagged either with Biotin or Digoxigenin using the unique ZytoFast HighTag System providing improved signal intensity! High specificity without risk of cross-hybridizations is obtained due to optimized oligonucleotide probes.
Advantages of Fast-CISH
- Simultaneous observation of tissue morphology and CISH signals
- No risk of false positives due to mispriming or contamination as with PCR
- Easy method comparable to IHC
- No costly equipment needed
- Ability to test archival specimens
- High sensitivity and specificity
ZytoFast – Flexibility that meets your Needs
Several ZytoFast and ZytoFast PLUS CISH Implementation Kits including different enzyme/substrate combinations can be combined with any separately available Biotin-labeled ZytoFast probe and Digoxigenin-labeled ZytoFast probe, respectively, to meet your preferences concerning the detection chemistry, counterstaining, and embedding. Each ZytoFast and ZytoFast PLUS CISH Implementation Kit includes a detailed protocol, all necessary reagents as well as positive and negative control probes for versatile use in DNA as well as RNA in situ Hybridizations.
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