Reliable and Simple Detection of Genomic Alterations Using Light Microscopy!
The ZytoDot ® and the ZytoDot ® 2C™ products are designed for the detection of aneuploidies, gene amplifications or gene deletions and translocations by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.
Method Description – ZytoDot
Single Color CISH for the Detection of Genomic Alterations
The ZytoDot system uses Digoxigenin-labeled probes (1) which are, after blocking (2), detected using a Mouse-anti-Digoxigenin antibody (3). This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody (4). The enzymatic reaction of DAB (5) leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.
MethodDescription – ZytoDot 2C
The ZytoDot 2C™ system uses DIG- and DNP-labeled probe cocktails targeting different genomic sections (1) which are detected using a Mouse-anti-DIG/Rabbit-anti-DNP cocktail (2). These antibodies are detected by a unique cocktail of polymerized HRP-Goat-anti-Mouse/AP-Goat-anti-Rabbit antibodies (3). The enzymatic reaction of AP-Red (4) and HRP-Green (5) leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy using a 40x objective.
Advantages of CISH over FISH
• Quick and easy interpretation of results comparable to IHC
• Simultaneous observation of tissue morphology and CISH signals
• Storage of slides at room temperature – CISH signals are permanent
• No costly fluorescent microscope needed.
High Signal-to-Noise Ratio
The ZytoDot® probes are processed by the unique ZytoVision®Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!
ZytoDot Kits and ZytoDot 2C Kits – Convenient Solutions
For making CISH analysis reliable and user-friendly, all ZytoDot® CISH probes can be combined with the ZytoDot®CISH Implementation Kit (C-3018-40) which includes all necessary pretreatment solutions, wash buffers, antibodies, chromogenic substrates, counterstaining solution, mounting solution and a detailed protocol to perform successful CISH experiments. Additionally, for some major targets, complete kits including probes and all necessary reagents are available.
|C-3001-400||ZytoDot SPEC HER2 Probe||400 μl|
|C-3002-400||ZytoDot CEN 6 Probe||400 μl|
|C-3006-400||ZytoDot CEN 17 Probe||400 μl|
|C-3007-400||ZytoDot SPEC EGFR Probe||400 μl|
|C-3008-400||ZytoDot CEN 7 Probe||400 μl|
|C-3012-400||ZytoDot SPEC MDM2 Probe||400 μl|
|C-3013-400||ZytoDot SPEC CMYC Probe||400 μl|
|C-3014-400||ZytoDot CEN 12 Probe||400 μl|
|C-3016-400||ZytoDot CEN 8 Probe||400 μl|
|C-3020-400||ZytoDot CEN Yq12 Probe||400 μl|
|C-3025-400||ZytoDot CEN X Probe||400 μl|
|C-3026-400||ZytoDot SPEC 21q22 Probe||400 μl|
|C-3029-400||ZytoDot SPEC NMYC Probe||400 μl|
|C-3035-400||ZytoDot SPEC 1p12 Probe||400 μl|
|C-3045-400||ZytoDot CEN 3 Probe||400 μl|
|C-3051-400||ZytoDot SPEC 2q11 Probe||400 μl|
|C-3052-400||ZytoDot SPEC 13q12 Probe||400 μl|