HER-2 neu ZytoDot CISH Probe on an FFPE Breast Carcinoma
FGR1 ZytoDot CISH Probe on an FFPE Prostate Carcinoma
TOPO2A ZytoDot CISH Probe on an FFPE Breast Carcinoma
Reliable and Simple Detection of Genomic Alterations Using Light Microscopy!
Introduction
The ZytoDot ® and the ZytoDot ® 2C™ products are designed for the detection of aneuploidies, gene amplifications or gene deletions and translocations by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.
Method Description – ZytoDot
Single Color CISH for the Detection of Genomic Alterations
The ZytoDot system uses Digoxigenin-labeled probes (1) which are, after blocking (2), detected using a Mouse-anti-Digoxigenin antibody (3). This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody (4). The enzymatic reaction of DAB (5) leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.
MethodDescription – ZytoDot 2C
The ZytoDot 2C™ system uses DIG- and DNP-labeled probe cocktails targeting different genomic sections (1) which are detected using a Mouse-anti-DIG/Rabbit-anti-DNP cocktail (2). These antibodies are detected by a unique cocktail of polymerized HRP-Goat-anti-Mouse/AP-Goat-anti-Rabbit antibodies (3). The enzymatic reaction of AP-Red (4) and HRP-Green (5) leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy using a 40x objective.
Advantages of CISH over FISH
• Quick and easy interpretation of results comparable to IHC
• Simultaneous observation of tissue morphology and CISH signals
• Storage of slides at room temperature – CISH signals are permanent
• No costly fluorescent microscope needed.
High Signal-to-Noise Ratio
The ZytoDot® probes are processed by the unique ZytoVision®Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!
ZytoDot Kits and ZytoDot 2C Kits – Convenient Solutions
For making CISH analysis reliable and user-friendly, all ZytoDot® CISH probes can be combined with the ZytoDot®CISH Implementation Kit (C-3018-40) which includes all necessary pretreatment solutions, wash buffers, antibodies, chromogenic substrates, counterstaining solution, mounting solution and a detailed protocol to perform successful CISH experiments. Additionally, for some major targets, complete kits including probes and all necessary reagents are available.
CAT.# | PRESENTATION | VOL/QTY |
| C-3001-400 | ZytoDot SPEC HER2 Probe | 400 μl |
| C-3002-400 | ZytoDot CEN 6 Probe | 400 μl |
| C-3006-400 | ZytoDot CEN 17 Probe | 400 μl |
| C-3007-400 | ZytoDot SPEC EGFR Probe | 400 μl |
| C-3008-400 | ZytoDot CEN 7 Probe | 400 μl |
| C-3012-400 | ZytoDot SPEC MDM2 Probe | 400 μl |
| C-3013-400 | ZytoDot SPEC CMYC Probe | 400 μl |
| C-3014-400 | ZytoDot CEN 12 Probe | 400 μl |
| C-3016-400 | ZytoDot CEN 8 Probe | 400 μl |
| C-3020-400 | ZytoDot CEN Yq12 Probe | 400 μl |
| C-3025-400 | ZytoDot CEN X Probe | 400 μl |
| C-3026-400 | ZytoDot SPEC 21q22 Probe | 400 μl |
| C-3029-400 | ZytoDot SPEC NMYC Probe | 400 μl |
| C-3035-400 | ZytoDot SPEC 1p12 Probe | 400 μl |
| C-3045-400 | ZytoDot CEN 3 Probe | 400 μl |
| C-3051-400 | ZytoDot SPEC 2q11 Probe | 400 μl |
| C-3052-400 | ZytoDot SPEC 13q12 Probe | 400 μl |
CAT.# | PRESENTATION | VOL/QTY |
CAT.# | PRESENTATION | VOL/QTY |
