CISH-HER2

CISH of HER-2 neu on an FFPE Breast Carcinoma

CISH-FGR1

CISH of FGR1 on an FFPE Prostate Carcinoma

CISH-TOPO2A

CISH of TOPO2A on an FFPE Breast Carcinoma

Reliable and Simple Detection of Genomic Alterations Using Light Microscopy!

Introduction

The ZytoDot ® and the ZytoDot ® 2C™ products are designed for the detection of aneuploidies, gene amplifications or gene deletions and translocations by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.

Method Description – ZytoDot

Single Color CISH for the Detection of Genomic Alterations

zytodot_method_smallThe ZytoDot system uses Digoxigenin-labeled probes (1) which are, after blocking (2), detected using a Mouse-anti-Digoxigenin antibody (3). This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody (4). The enzymatic reaction of DAB (5) leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.

MethodDescription – ZytoDot 2C

zytodot_2c_method_small 2

The ZytoDot 2C™ system uses DIG- and DNP-labeled probe cocktails targeting different genomic sections (1) which are detected using a Mouse-anti-DIG/Rabbit-anti-DNP cocktail (2). These antibodies are detected by a unique cocktail of polymerized HRP-Goat-anti-Mouse/AP-Goat-anti-Rabbit antibodies (3). The enzymatic reaction of AP-Red (4) and HRP-Green (5) leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy using a 40x objective.

Advantages of CISH over FISH

• Quick and easy interpretation of results comparable to IHC
• Simultaneous observation of tissue morphology and CISH signals
• Storage of slides at room temperature – CISH signals are permanent
• No costly fluorescent microscope needed.

High Signal-to-Noise Ratio

The ZytoDot® probes are processed by the unique ZytoVision®Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!

ZytoDot Kits and ZytoDot 2C Kits – Convenient Solutions

For making CISH analysis reliable and user-friendly, all ZytoDot® CISH probes can be combined with the ZytoDot®CISH Implementation Kit (C-3018-40) which includes all necessary pretreatment solutions, wash buffers, antibodies, chromogenic substrates, counterstaining solution, mounting solution and a detailed protocol to perform successful CISH experiments. Additionally, for some major targets, complete kits including probes and all necessary reagents are available.

ZytoDot Single Color Probes

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VOL/QTY

ZytoDot Dual Color Probes

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PRESENTATION
VOL/QTY
 C-3032-100  ZytoDot 2C SPEC HER2/CEN 17 Probe  100 μl
 C-3032-400  ZytoDot 2C SPEC HER2/CEN 17 Probe  400 μl
 C-3033-100  ZytoDot 2C SPEC EGFR/CEN 7 Probe  100 μl
C-3033-400 ZytoDot 2C SPEC EGFR/CEN 7 Probe 400 μl
C-3036-100 ZytoDot 2C SPEC 1p36/1q25  100 μl
C-3036-400 ZytoDot 2C SPEC 1p36/1q25  400 μl
C-3037-100 ZytoDot 2C SPEC 19q13/19p13 100 μl
C-3037-400 ZytoDot 2C SPEC 19q13/19p13  400 μl
C-3040-400 ZytoDot 2C SPEC TOP2A/CEN 17 Probe  400 μl
C-3043-100  ZytoDot 2C SPEC EWSR1 Break Apart Probe  100 μl
C-3046-100 ZytoDot 2C SPEC SYT Break Apart Probe  100 μl
C-3047-100 ZytoDot 2C SPEC CHOP Break Apart Probe  100 μl
 C-3048-400 ZytoDot 2C CEN X/Y Probe  400 μl
C-3049-100 ZytoDot 2C SPEC MDM2/CEN12 Probe  100 μl
C-3049-400  ZytoDot 2C SPEC MDM2/CEN12 Probe  400 μl
C-3050-400 ZytoDot 2C SPEC FGFR1/CEN8 Probe  400 μl
 C-3053-400  ZytoDot 2C SPEC PTEN/CEN10 Probe  400 μl
 C-3054-100 ZytoDot 2C SPEC FUS Break Apart Probe  100 μl
 C-3055-100 ZytoDot 2C SPEC ALK Break Apart Probe  100 μl
 C-3055-400 ZytoDot 2C SPEC ALK Break Apart Probe  400 μl
 C-3056-400 ZytoDot 2C SPEC FGFR2/CEN 10 Probe  400 μl
 C-3057-400 ZytoDot 2C SPEC MET/CEN 7 Probe  400 μl
 C-3058-400  ZytoDot 2C SPEC ERG Break Apart Probe  400 μl
 C-3059-400  ZytoDot 2C SPEC EML4 Break Apart Probe  400 μl
 C-3062-400  ZytoDot 2C SPEC CDK4/CEN 12 Break Apart Probe  400 μl
 C-3063-100  ZytoDot 2C SPEC ROS1 Break Apart Probe  100 μl
 C-3063-400  ZytoDot 2C SPEC ROS1 Break Apart Probe  400 μl
 C-3064-100  ZytoDot 2C SPEC RET Break Apart Probe  100 μl
 C-3064-400  ZytoDot 2C SPEC RET Break Apart Probe  400 μl
 C-3065-100  ZytoDot 2C SPEC FOXO1 Break Apart Probe  100 μl
 C-3066-400  ZytoDot 2C SPEC MYC Break Apart Probe  400 μl
 C-3067-400  ZytoDot 2C SPEC CDKN2A/CEN 9 Probe  400 μl
 C-3068-100  ZytoDot 2C SPEC ERBB2/D17S122 Probe  100 μl
 C-3069-400  ZytoDot 2C SPEC TOP1/CEN20 Probe  400 μl
 C-3071-100  ZytoDot 2C SPEC IGH Break Apart Probe Probe  100 μl
 C-3072-100  ZytoDot 2C SPEC MALT1 Break Apart Probe  100 μl
 C-3073-100  ZytoDot 2C SPEC BCL2 Break Apart Probe  100 μl
 C-3074-100  ZytoDot 2C SPEC BCL6 Break Apart Probe  100 μl
 C-3075-100  ZytoDot 2C SPEC CCND1 Break Apart Probe  100 μl

ZytoDot CISH Kits

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