HER-2 neu ZytoLight FlexISH Probe on an FFPE Breast Carcinoma
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FlexISH – Reliable ERBB2 Results Obtainable in 1 Day!
Introduction
ZytoLight FlexISH products are designed for identification of chromosomal aberrations on various specimens by Fluorescence in situ Hybridization (FISH). Using the FlexISH products gives you the flexibility to choose between a 1-day (2 h hybridization) or a 2-day (overnight hybridization) protocol by adapting the hybridization time just according to your individual needs!
FlexISH Brings Flexibility to Your FISH
- FlexISH maximizes your flexibility in terms of time and laboratory management. Hybridization time can be varied between 2 hours and overnight
- With a hybridization temperature of 37°C the FlexISH protocol is fully compatible with routine workflows in pathology laboratories.
- In a rcent comparison study, overnight and short hybridization periods showed excellent correlation between the FISH results obtained with FlexISH, ZytoLight, and PathVysion ERBB2 probes.
- Short hybridization time does not negatively affect the performance, specimen quality or diagnostic results.
Method Description & FlexISH Fluorochromes
The FlexISH system uses direct labeled FISH probes (1). The probes are detected by fluorescence microscopy using appropriate filter sets (2). Due to an exciter filter (3), full-spectrum light, emitted by the microscope lamp (4), is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror (5). The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter (6) reduces the emitted light to a defined wavelength that can be detected.
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