FISH-HER2

FISH of HER-2 neu on an FFPE Breast Carcinoma

FISH-EGFR

FISH of EGFR on an FFPE Colon Carcinoma

FISH-MDM2

FISH of MDM2 on an FFPE Liposcarcinoma

Reliable multi-target detection using Fluorescence in situ Hybridization!

Introduction

ZytoLight® products are designed for the identification of genetic aberrations e.g. translocations, deletions, amplifications, and chromosomal aneuploidies by Fluorescence in situ Hybridization (FISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.

Method Description

Zytolight_method

The ZytoLight system uses direct labeled FISH probes (1), eliminating the need to detect the probes with fluorophore-coupled antibodies. The probes are detected by fluorescence microscopy using appropriate filter sets (2). Due to an exciter filter (3), full-spectrum light, emitted by the microscope lamp (4), is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror (5). The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter (6) reduces the emitted light to a defined wavelength that can be detected.

High Sensitivity and Specificity

ZytoLight® FISH probes are directly labeled using the unique ZytoLight® Direct Label System II providing improved signal intensity. All ZytoLight® single copy (SPECTM) probes are processed by the unique ZytoLight® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!

ZytoLight® CENTM probes hybridize to highly repetitive human satellite DNA sequences located at the centromeric regions of chromosomes producing sharp, bright signals specific for each individual chromosome.   

ZytoLight® Kits – Convenient Solutions

For making FISH analysis reliable and user-friendly, all ZytoLight FISH probes can be combined with the ZytoLight FISH-Tissue Implementation Kit (Z-2028-20) for FISH analyses on tissue specimens. Implementation Kits include all necessary pretreatment solutions, wash buffers and DAPI/Antifade solution and a detailed protocol to perform successful FISH experiments. Additionally, for some major targets, complete kits including probes and all necessary reagents are available.

ZytoLight Single Color Probes

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ZytoLight Dual Color Probes

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PRESENTATION
VOL/QTY
 Z-2013-200 ZytoLight SPEC MDM2/CEN 12 Dual Color Probe  200 μl
 Z-2013-50 ZytoLight SPEC MDM2/CEN 12 Dual Color Probe  50 μl
 Z-2014-200  ZytoLight SPEC MAML2 Dual Color Break Apart Probe 200 μl
 Z-2014-50  ZytoLight SPEC MAML2 Dual Color Break Apart Probe 50 μl
Z-2015-200  ZytoLight SPEC HER2/CEN 17 Dual Color Probe  200 μl
 Z-2015-50 ZytoLight SPEC HER2/CEN 17 Dual Color Probe  50 μl
Z-2016-200 ZytoLight CEN X/Yq12 Dual Color Probe  200 μl
Z-2016-50 ZytoLight CEN X/Yq12 Dual Color Probe  50 μl
Z-2018-200 ZytoLight SPEC FOXO1/PAX3 Dual Color Single Fusion Probe  200 μl
 Z-2018-50 ZytoLight SPEC FOXO1/PAX3 Dual Color Single Fusion Probe  50 μl
 Z-2019-200 ZytoLight SPEC FOXO1/PAX7 Dual Color Single Fusion Probe  200 μl
 Z-2019-50 ZytoLight SPEC FOXO1/PAX7 Dual Color Single Fusion Probe  50 μl
 Z-2020-200 ZytoLight SPEC ERBB2/CEN 17 Dual Color Probe Kit  200 μl
 Z-2020-50 ZytoLight SPEC ERBB2/CEN 17 Dual Color Probe Kit  50 μl
Z-2033-200  ZytoLight SPEC EGFR/CEN 7 Dual Color Probe  200 μl
Z-2033-50 ZytoLight SPEC EGFR/CEN 7 Dual Color Probe  50 μl
Z-2056-200  ZytoLight SPEC HER3/CEN 12 Dual Color Probe  200 μl
Z-2057-200 ZytoLight SPEC HER4/2q11 Dual Color Probe  200 μl
Z-2062-200  ZytoLight SPEC FHIT/CEN 3 Dual Color Probe  200 μl
 Z-2063-200  ZytoLight SPEC p16/CEN 9 Dual Color Probe  200 μl
 Z-2063-50  ZytoLight SPEC p16/CEN 9 Dual Color Probe  50 μl
 Z-2069-200 ZytoLight SPEC ESR1/CEN 6 Dual Color Probe  200 μl
Z-2069-50  ZytoLight SPEC ESR1/CEN 6 Dual Color Probe  50 μl
 Z-2071-200 ZytoLight SPEC CCND1/CEN 11 Dual Color Probe  200 μl
 Z-2071-50 ZytoLight SPEC CCND1/CEN 11 Dual Color Probe  50 μl
 Z-2072-200 ZytoLight SPEC FGFR1/CEN 8 Dual Color Probe  200 μl
 Z-2072-50 ZytoLight SPEC FGFR1/CEN 8 Dual Color Probe  50 μl
Z-2074-200 ZytoLight SPEC NMYC/2q11 Dual Color Probe  200 μl
Z-2074-50 ZytoLight SPEC NMYC/2q11 Dual Color Probe  50 μl
Z-2075-200 ZytoLight SPEC 1p36/1q25 Dual Color Probe  200 μl
Z-2075-50 ZytoLight SPEC 1p36/1q25 Dual Color Probe  50 μl
 Z-2076-200 ZytoLight SPEC 19q13/19p13 Dual Color Probe  200 μl
Z-2076-50 ZytoLight SPEC 19q13/19p13 Dual Color Probe  50 μl
Z-2077-200 ZytoLight SPEC CEN 17/SPEC ERBB2 Dual Color Probe  200 μl
Z-2077-50 ZytoLight SPECCEN 17/SPEC ERBB2 Dual Color Probe  50 μl
 Z-2078-200 ZytoLight SPEC PTEN/CEN 10 Dual Color Probe  200 μl
 Z-2078-50 ZytoLight SPEC PTEN/CEN 10 Dual Color Probe  50 μl
 Z-2080-200  ZytoLight SPEC MDM4/1p12 Dual Color Probe  200 μl
 Z-2082-200  ZytoLight SPEC FGFR3/CEN 4 Dual Color Probe  200 μl
 Z-2084-200 ZytoLight SPEC VHL/CEN 3 Dual Color Probe  200 μl
Z-2087-200 ZytoLight SPEC MET/CEN 7 Dual Color Probe  200 μl
Z-2090-200 ZytoLight SPEC CMYC Dual Color Break Apart Probe  200 μl
Z-2090-50 ZytoLight SPEC CMYC Dual Color Break Apart Probe  50 μl
Z-2091-200 ZytoLight SPEC TERT/5q31 Dual Color Probe  200 μl
Z-2092-200  ZytoLight SPEC CMYC/CEN 8 Dual Color Probe  200 μ
Z-2092-50  ZytoLight SPEC CMYC/CEN 8 Dual Color Probe  50 μ
Z-2094-200 ZytoLight porcine X/Y Dual Color Probe  200 μl
 Z-2096-200 ZytoLight SPEC EWSR1 Dual Color Break Apart Probe  200 μl
 Z-2096-50 ZytoLight SPEC EWSR1 Dual Color Break Apart Probe  50 μl
 Z-2097-200 ZytoLight SPEC SYT Dual Color Break Apart Probe  200 μl
 Z-2097-50 ZytoLight SPEC SYT Dual Color Break Apart Probe  50 μl
Z-2098-200 ZytoLight SPEC TYMS/CEN 18 Dual Color Probe  200 μl
Z-2100-200 ZytoLight SPEC CHOP Dual Color Break Apart Probe  200 μl
Z-2100-50 ZytoLight SPEC CHOP Dual Color Break Apart Probe  50 μl
Z-2103-200 ZytoLight SPEC CDK4/CEN 12 Dual Color Probe  200 μl
Z-2103-50 ZytoLight SPEC CDK4/CEN 12 Dual Color Probe  50 μl
Z-2104-10 ZytoLight Aneusomy Probe Set Dual Color Probe  2×50 μl
Z-2104-40 ZytoLight Aneusomy Probe Set Dual Color Probe 2×200 μl
Z-2105-200  ZytoLight SPEC CMYC/IGH Dual Color Dual Fusion Probe  200 μl
Z-2105-50  ZytoLight SPEC CMYC/IGH Dual Color Dual Fusion Probe  50 μl
Z-2107-200 ZytoLight SPEC EGR1/5p15 Dual Color Probe  200 μl
Z-2107-50 ZytoLight SPEC EGR1/5p15 Dual Color Probe  50 μl
Z-2108-200 ZytoLight SPEC CCND1 Dual Color Break Apart Probe  200 μl
Z-2108-50 ZytoLight SPEC CCND1 Dual Color Break Apart Probe  50 μl
Z-2109-200 ZytoLight SPEC TFE3 Dual Color Break Apart Probe  200 μl
Z-2109-50 ZytoLight SPEC TFE3 Dual Color Break Apart Probe  50 μl
Z-2110-200 ZytoLight SPEC IGH Dual Color Break Apart Probe  200 μl
Z-2110-50 ZytoLight SPEC IGH Dual Color Break Apart Probe  200 μl
Z-2111-200 ZytoLight SPEC BCR/ABL Dual Color Dual Fusion Probe 200 μl
Z-2111-50 ZytoLight SPEC BCR/ABL Dual Color Dual Fusion Probe 50 μl
Z-2112-50 ZytoLight SPEC RUNX1/RUNX1T1 Dual Color Dual Fusion Probe 50 μl
Z-2113-200 ZytoLight SPEC PML/RARA Dual Color Dual Fusion Probe 200 μl
Z-2113-50 ZytoLight SPEC PML/RARA Dual Color Dual Fusion Probe 50 μl
 Z-2114-200 ZytoLight SPEC BCL2/IGH Dual Color Dual Fusion Probe  200 μl
 Z-2114-50 ZytoLight SPEC BCL2/IGH Dual Color Dual Fusion Probe  50 μl
 Z-2115-200 ZytoLight SPEC KRAS/CEN 12 Dual Color Dual Fusion Probe  200 μl
 Z-2116-200 ZytoLight SPEC COL1A1/PDGFB Dual Color Dual Fusion Probe  200 μl
 Z-2116-50 ZytoLight SPEC COL1A1/PDGFB Dual Color Dual Fusion Probe  200 μl
 Z-2119-200  ZytoLight SPEC PDGFB Dual Color Break Apart Probe  200 μl
 Z-2119-50  ZytoLight SPEC PDGFB Dual Color Break Apart Probe  50 μl
 Z-2120-200 ZytoLight CEN X/Y Dual Color Probe  200 μl
 Z-2121-200 ZytoLight SPEC COL1A1 Dual Color Break Apart Probe  200 μl
 Z-2122-200 ZytoLight SPEC FGFR2/CEN 10 Dual Color Probe  200 μl
 Z-2123-200 ZytoLight SPEC CEN Y (DYZ3) Probe  200 μl
Z-2124-200  ZytoLight SPEC ALK Dual Color Break Apart Probe  200 μl
Z-2124-50 ZytoLight SPEC ALK Dual Color Break Apart Probe  50 μl
Z-2125-200 ZytoLight SPEC CCND1/IGH Dual Color Dual Fusion Probe  200 μl
Z-2125-50 ZytoLight SPEC CCND1/IGH Dual Color Dual Fusion Probe  50 μl
Z-2127-200 ZytoLight SPEC SOX2/CEN 3 Dual Color Probe  200 μl
Z-2130-50 ZytoLight SPEC FUS Dual Color Break Apart Probe 50 μl
Z-2131-50 ZytoLight SPEC KIF5B Dual Color Break Apart Probe 50 μl
Z-2132-50 ZytoLight SPEC JAXF1 Dual Color Break Apart Probe 50 μl
Z-2133-50 ZytoLight SPEC TFG Dual Color Break Apart Probe 50 µl
Z-2136-50 ZytoLight SPEC EML4 Dual Color Break Apart Probe 50 µl
Z-2137-50 ZytoLight SPEC CARS Dual Color Break Apart Probe 50 µl
Z-2138-200 ZytoLight SPEC ERG Dual Color Break Apart Probe 200 µl
Z-2139-50 ZytoLight SPEC FOX01 Dual Color Break Apart Probe 50 µl
Z-2140-200 ZytoLight SPEC PIK3CA/CEN 3 Dual Color Break Apart Probe 200 µl
Z-2141-200 ZytoLight SPEC ETV1/CEN 7 Dual Color Probe 200 μl
Z-2142-50 ZytoLight SPEC WT1 Dual Color Probe 50 μl
Z-2143-200 ZytoLight SPEC MYB Dual Color Probe 200 μl
Z-2144-50 ZytoLight SPEC ROS1 Dual Color Break Apart Probe 50 µl
Z-2144-200 ZytoLight SPEC ROS1 Dual Color Break Apart Probe 200 µl
Z-2145-50 ZytoLight SPEC NR4A3 Dual Color Break Apart Probe 50 µl
Z-2146-200 ZytoLight SPEC BIRC3/MALT1 Dual Color Dual Fusion Probe 200 µl
Z-2146-50 ZytoLight SPEC BIRC3/MALT1 Dual Color Dual Fusion Probe 50 µl
Z-2148-200 ZytoLight SPEC RET Dual Color Break Apart Probe 200 µl
Z-2148-50 ZytoLight SPEC RET Dual Color Break Apart Probe 50 µl
Z-2151-50 ZytoLight SPEC USP6 Dual Color Break Apart Probe 50 µl
Z-2153-200 ZytoLight SPEC TP53/CEN 17 Dual Color Probe 200 µl
Z-2153-50 ZytoLight SPEC TP53/CEN 17 Dual Color Probe 50 µl
Z-2154-200 ZytoLight SPEC AXL/19p13 Dual Color Probe 200 µl
Z-2155-200 ZytoLight SPEC MERTK/2q11 Dual Color Probe 200 µl
Z-2156-200 ZytoLight SPEC GAS6/13q12 Dual Color Probe 200 µl
Z-2157-200 ZytoLight SPEC ETV6/RUNX1 Dual Color Fuison Probe 200 µl
Z-2158-200 New! ZytoLight SPEC FGF3,4,19/CEN11 Dual Color Break Apart Probe 200 µl
Z-2159-200 ZytoLight CLL I Probe SPEC TP53/ATM 200 µl
Z-2159-50 ZytoLight CLL I Probe SPEC TP53/ATM 50 µl
Z-2161-200 ZytoLight SPEC ALK/2q11 Dual Color Probe 200 µl
Z-2162-200 ZytoLight SPEC ROS1/CEN6 Dual Color Probe 200 µl
Z-2164-200 New! ZytoLight SPEC 13/21 Dual Color Break Apart Probe 200 µl
Z-2165-200 ZytoLight SPEC RB1/13q12 Dual Color Probe 200 µl
Z-2165-50 ZytoLight SPEC RB1/13q12 Dual Color Probe 50 µl
Z-2167-200 ZytoLight SPEC NTRK1 Dual Color Break Apart Probe 200 µl
Z-2168-200 ZytoLight SPEC FGFR1 Dual Color Break Apart Probe 200 µl
Z-2168-50 ZytoLight SPEC FGFR1 Dual Color Break Apart Probe 50 µl
Z-2169-200 ZytoLight SPEC FGFR2 Dual Color Break Apart Probe 200 µl
Z-2170-200 ZytoLight SPEC FGFR3 Dual Color Break Apart Probe 200 µl
Z-2170-50 ZytoLight SPEC FGFR3 Dual Color Break Apart Probe 50 µl
Z-2171-200 ZytoLight SPEC BCL2L1/CEN 20 Dual Color Probe 200 µl
Z-2172-200 ZytoLight SPEC BCL2L1/14q32 Dual Color Probe 200 µl
Z-2173-200 ZytoLight SPEC MCL1/1p12 Dual Color Probe 200 µl
Z-2174-50 New! ZytoLight SPEC BCL2/CEN 18 Dual Color Probe 50 µl
Z-2175-50 New! ZytoLight SPEC YWHAE Dual Color Break Apart Probe 50 µl
Z-2176-200 New! ZytoLight SPEC ETV6 Dual Color Break Apart Probe 200 µl
Z-2176-50 New! ZytoLight SPEC ETV6 Dual Color Break Apart Probe 50 µl
Z-2177-200 New! ZytoLight SPEC BCL6 Dual Color Break Apart Probe 200 µl
Z-2177-50 New! ZytoLight SPEC BCL6 Dual Color Break Apart Probe 50 µl
Z-2178-50 New! ZytoLight SPEC SMARCB1/22q12 Dual Color Probe 50 µl
Z-2179-200 New! ZytoLight SPEC CD274 PDCD1LG2CEN 9 Dual Color Probe 200 µl
Z-2179-50 New! ZytoLight SPEC CD274 PDCD1LG2CEN 9 Dual Color Probe 50 µl
Z-2181-200 New! ZytoLight SPEC NRG1 Dual Color Probe 200 µl
Z-2189-200 New! ZytoLight SPEC BRAF Dual Color Probe 200 µl
Z-2190-50 New! ZytoLight SPEC ERBB2/D17S122 Dual Color Probe 50 µl
Z-2191-200 New! ZytoLight SPEC BRAF/CEN 7 Dual Color Probe 200 µl
Z-2192-200 New! ZytoLight SPEC BCL2 Dual Color Break Apart Probe 200 µl
Z-2192-50 New! ZytoLight SPEC BCL2 Dual Color Break Apart Probe 50 µl
Z-2193-50 New! ZytoLight SPEC KMT2A Dual Color Break Apart Probe 50 µl
Z-2195-200 New! ZytoLight SPEC VEGFA/CEN 6 Dual Color Break Apart Probe 200 µl
Z-2196-200 ZytoLight SPEC MALT1 Dual Color Break Apart Probe 200 µl
Z-2196-50 ZytoLight SPEC MALT1 Dual Color Break Apart Probe 50 µl
Z-2197-200 New! ZytoLight SPEC PDGFRB Dual Color Break Apart Probe 200 µl
Z-2198-200 New! ZytoLight SPEC TP53/17q22 Dual Color Break Apart Probe 200 µl
Z-2199-200 New! ZytoLight SPEC ABL1 Dual Color Break Apart Probe 200 µl
Z-2200-200 New! ZytoLight SPEC ABL2 Dual Color Break Apart Probe 200 µl
Z-2201-200 New! ZytoLight SPEC CRLF2 Dual Color Break Apart Probe 200 µl
Z-2202-200 New! ZytoLight SPEC CSF1R Dual Color Break Apart Probe 200 µl
Z-2205-200 New! ZytoLight SPEC NTRK2 Dual Color Break Apart Probe 200 µl
Z-2206-200 New! ZytoLight SPEC NTRK3 Dual Color Break Apart Probe 200 µl
Z-2207-50 New! ZytoLight SPEC CBFB Dual Color Break Apart Probe 50 µl
Z-2208-200 New! ZytoLight SPEC NUTM1 Dual Color Break Apart Probe 200 µl
Z-2210-50 ZytoLight SPEC IRF4,DUSP22 Dual Color Break Apart Probe 50 µl
Z-2211-50 ZytoLight SPEC EGR1/D5S23,D5S721 Dual Color Probe 50 µl
Z-2212-50 ZytoLight SPEC WWTR1 Dual Color Break Apart Probe 50 µl
Z-2213-50 ZytoLight SPEC PTPRT/20q11 Dual Color Probe 50 µl
Z-2215-50 ZytoLight SPEC PHF1 Dual Color Break Apart Probe 50 µl
Z-2265-50 ZytoLight SPEC NUP214 Dual Color Break Apart Probe 50 µl
Z-2266-50 ZytoLight SPEC NUP98 Dual Color Break Apart Probe 50 µl
Z-2267-50 ZytoLight SPEC CREBBP Dual Color Break Apart Probe 50 µl

ZytoLight Triple Color Probes

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ZytoLight Quad Color Probes

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ZytoLight FISH Kits

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