{"type":"root","children":[{"type":"paragraph","children":[{"type":"text","value":"The BOB-1 protein is a co-activator that interacts with Oct1 and/or Oct2 transcription factors, and is critical in germinal center formation and immunoglobulin production. The strongest expression of BOB-1 is found in the germinal center, mantle-zone B cells, and plasma cells. Because BOB-1/OBF.1 are germinal center derived, L&H cells in Nodular Lymphocyte Predominant Hodgkin Lymphoma are consistently immunoreactive for BOB-1. Conversely, the Hodgkin/Reed-Sternberg cells in classical Hodgkin Lymphoma either do not express both or express only one of the two proteins."}]},{"type":"paragraph","children":[{"type":"text","value":"In Diffuse Large B-cell Lymphomas, the highest expression levels for BOB-1/OBF.1 are reported in Follicular Center Lymphomas, Diffuse Large B-cell Lymphomas, and Burkitt Lymphomas. B-CLL, MALT-type, and Mantle Cell Lymphomas score negative or display a heterogenous/weaker activity. The strong nuclear expression of BOB-1 and Oct-2 by Germinal Center Derived Lymphomas makes these antibodies a novel class of broad spectrum B-lineage immunohistochemical markers in the differential diagnosis of Lymphomas, specifically between Primary Mediastinal B-cell Lymphoma from classical Hodgkin Disease."}]}]}