| Intended Use | For In Vitro Diagnostic Use | |||||||||||
| Summary and Explanation | HPV infection is commonly found in Squamous Cell Carcinomas (SCC) of the head, neck, and female genital tract. High-risk HPV was found in the lesions of grade 1, 2, and 3 Cervical Intraepithelial Neoplasia (CIN), the precursor to cervical cancer. HPV genome integration causes increased expression of tumor suppressor gene p16, which can correlate roughly to the degree of cervical dysplasia. Colocalized Ki67 and p16 help identify CIN lesions and SCC for proper diagnosis and prevention of under-or over-treatment. p16 overexpression is commonly used to detect squamous and glandular neoplasia in HPV-positive patients. Ki67 is a nuclear cell proliferation marker, applied to diagnose the grade of abnormal cell growth. The HPV MultiDetector HRP/AP Kit dual stain includes a cocktail of Ki67 and p16 antibodies to form a sensitive and specific test for significant cervical lesions or oral and oropharyngeal SCC. Detection of nuclear Ki67 with DAB chromogen and nuclear and cytoplasmic p16 with ALK Scarlet provides an easy visualization of the colocalized antibodies for efficient diagnosis on biopsies and liquid cytology samples. | |||||||||||
| Presentation | The HPV MultiDetector HRP/AP Kit contains a predilute rabbit monoclonal Ki67 (RM360) and mouse monoclonal p16 (16P04, JC2) antibody cocktail, ImmunoDNA Retriever with Citrate heat epitope retrieval solution, ImmunoDNA Washer solution, Peroxidase Blocker solution, AP Blocker solution, a predilute Rabbit MultiDetector HRPand Mouse MultiDetector AP Label cocktail solution, a DAB Buffer and DAB Chromogen solution, an ALK Scarlet Buffer and Scarlet Chromogen solution, and a Fast ChromoProtector stain protection solution. All the components are buffered and contain proteins, stabilizers, and a non-azide antimicrobial. | |||||||||||
| Availability |
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| Components | Catalog No. | ||
BSB-0354-7 7 mL Kit 70 Tests* | BSB-0354-15 15 mL Kit 150 Tests* | BSB-0354-50 50 mL Kit 500 Tests* | |
| 20X ImmunoRetriever with Citrate | 50 mL | 100 mL | 200 mL |
| MultiDetector Peroxidase Blocker | 7 mL | 15 mL | 50 mL |
| MultiDetector AP Blocker | 7 mL | 15 mL | 50 mL |
| Ki67 RMab & p16 MMab Cocktail | 7 mL | 15 mL | 50 mL |
| Mouse MultiDetector AP & Rabbit MultiDetector HRP Label Cocktail | 7 mL | 15 mL | 50 mL |
| MultiDetector DAB Buffer-Substrate | 7 mL | 15 mL | 50 mL |
| MultiDetector DAB Chromogen | 1 mL | 2 mL | 6 mL |
| MultiDetector ALK Scarlet Buffer-Substrate | 7 mL | 15 mL | 50 mL |
| MultiDetector Scarlet Chromogen | 1 mL | 2 mL | 6 mL |
| 20X ImmunoDNA Washer | 50 mL | 100 mL | 200 mL |
| Fast ChromoProtector | 10 mL | 20 mL | 50 mL |
| Control Slides: 7-core HPV CLMA | 5 Slides | 10 Slides | 30 Slides |
Abbreviated Immunohistochemical Protocol
