cd14 anti-cd14 antibody immunohistochemistry ihc

CD14 Antibody Immunohistochemistry of CD14 on an FFPE Placenta Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and Explanation

CD14 is a component of the innate immune system. CD14 exists in two forms: mCD14 (anchored by a glycosylphosphatidylinositol tail) or sCD14 (soluble). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can only bind LPS in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered it’s main ligand, CD14 also recognizes other pathogen associated molecular patterns.

CD14 antibody is expressed mainly by macrophages and (at 10 times lesser extent) by neutrophil granulocytes. A soluble form sCD14 is secreted by the liver and monocytes and is sufficient in low concentrations to confer LPS-responsiveness to cells which otherwise do not express CD14. sCD14 is also present in human milk where it is believed to regulate microbial growth in the infant gut. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram-negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Antibody TypeRabbit MonoclonalCloneEP128
IsotypeIgGReactivityParaffin, Frozen
LocalizationCytoplasmic, MembranousControlPlacenta, Tonsil, Spleen, Diffuse Large B-cell Lymphoma
PresentationCD14 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 6457PredilutedReady-To-Use3.0 ml
BSB 6458PredilutedReady-To-Use7.0 ml
BSB 6459PredilutedReady-To-Use15.0 ml
BSB 6460Concentrated1:25-1:1000.1 ml
BSB 6461Concentrated1:25-1:1000.5 ml
BSB 6462Concentrated1:25-1:1001.0 ml
BSB 6463Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Synonyms: H4, H 4, anti-CD14, anti CD14, monocyte differentiation antigen CD14, CD14 marker, anti-monocyte differentiation antigen CD14, anti monocyte differentiation antigen CD14, anti-CD14 marker, anti CD14 marker