Simple and Reliable Chromogenic Detection of
Genomic Alterations Using Light Microscopy

 ZytoDot ® and ZytoDot ® 2C™ products are designed for the detection of aneuploidies, gene amplifications or gene deletions and translocations by chromogenic in situ hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears and metaphase chromosome spreads.

Detection Method
ZytoDot Single Color CISH

The ZytoDot system uses digoxigenin (DIG)-labeled probes which are detected using a mouse anti-DIG antibody. The antibody is then detected by a polymerized HRP-goat-anti-mouse antibody, facilitating the enzymatic reaction of DAB which forms strong, permanent brown staining that can be visualized by light microscopy.

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Detection Method
ZytoDot 2C

The ZytoDot 2C™ system uses digoxigenin (DIG) and dinitrophenyl (DNP)-labeled probe cocktails targeting different genomic sections which are detected using a mouse anti-DIG/rabbit anti-DNP cocktail. A unique cocktail of polymerized HRP-goat-anti-mouse/AP-goat-anti-rabbit antibodies creates an enzymatic reaction using AP Red and HRP Green. This forms strong permanent red and green signals that can be visualized by light microscopy.

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Advantages of [highlight]CISH[/highlight]

Advantages of CISH

  • Quick and easy interpretation of results compared to IHC
  • Simultaneous observation of tissue morphology and CISH signals
  • CISH signals are permanent and can be stored at room temperature
  • No costly fluorescent microscope needed
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High Signal-to-Noise Ratio

High Signal-to-Noise Ratio

The ZytoDot® probes are processed by the unique ZytoVision®Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed.
Convenient Kit Solutions

Convenient Kit Solutions

All ZytoDot® CISH probes can be combined with the ZytoDot®CISH Implementation Kit (C-3018-40). The kit includes all necessary pretreatment solutions, wash buffers, antibodies, chromogens, counterstaining solution, mounting solution and a detailed protocol, making CISH analysis reliable and user-friendly. For select targets, complete kits including probes and all necessary reagents are available.
CAT.# Presentation Vol/Qty
 C-3001-400 ZytoDot SPEC HER2 Probe 400 μl
 C-3002-400 ZytoDot CEN 6 Probe 400 μl
 C-3006-400 ZytoDot CEN 17 Probe 400 μl
C-3007-400 ZytoDot SPEC EGFR Probe 400 μl
C-3008-400 ZytoDot CEN 7 Probe 400 μl
C-3012-400 ZytoDot SPEC MDM2 Probe 400 μl
C-3013-400 ZytoDot SPEC CMYC Probe 400 μl
C-3014-400 ZytoDot CEN 12 Probe 400 μl
C-3016-400 ZytoDot CEN 8 Probe 400 μl
C-3020-400 ZytoDot CEN Yq12 Probe 400 μl
C-3025-400 ZytoDot CEN X Probe 400 μl
C-3026-400 ZytoDot SPEC 21q22 Probe 400 μl
C-3029-400 ZytoDot SPEC NMYC Probe 400 μl
C-3035-400 ZytoDot SPEC 1p12 Probe 400 μl
C-3045-400 ZytoDot CEN 3 Probe 400 μl
C-3051-400 ZytoDot SPEC 2q11 Probe 400 μl
C-3052-400 ZytoDot SPEC 13q12 Probe 400 μl
CAT.# Presentation Vol/Qty
C-3032-100 ZytoDot 2C SPEC HER2/CEN 17 Probe 100 μl
C-3032-400 ZytoDot 2C SPEC HER2/CEN 17 Probe 400 μl
C-3033-100 ZytoDot 2C SPEC EGFR/CEN 7 Probe 100 μl
C-3033-400 ZytoDot 2C SPEC EGFR/CEN 7 Probe 400 μl
C-3036-100 ZytoDot 2C SPEC 1p36/1q25 100 μl
C-3036-400 ZytoDot 2C SPEC 1p36/1q25 400 μl
C-3037-100 ZytoDot 2C SPEC 19q13/19p13 100 μl
C-3037-400 ZytoDot 2C SPEC 19q13/19p13 400 μl
C-3040-400 ZytoDot 2C SPEC TOP2A/CEN 17 Probe 400 μl
C-3043-100 ZytoDot 2C SPEC EWSR1 Break Apart Probe 100 μl
C-3046-100 ZytoDot 2C SPEC SYT Break Apart Probe 100 μl
C-3047-100 ZytoDot 2C SPEC CHOP Break Apart Probe 100 μl
C-3048-400 ZytoDot 2C CEN X/Y Probe 400 μl
C-3049-100 ZytoDot 2C SPEC MDM2/CEN12 Probe 100 μl
C-3049-400 ZytoDot 2C SPEC MDM2/CEN12 Probe 400 μl
C-3050-400 ZytoDot 2C SPEC FGFR1/CEN8 Probe 400 μl
C-3053-400 ZytoDot 2C SPEC PTEN/CEN10 Probe 400 μl
C-3054-100 ZytoDot 2C SPEC FUS Break Apart Probe 100 μl
C-3055-100 ZytoDot 2C SPEC ALK Break Apart Probe 100 μl
C-3055-400 ZytoDot 2C SPEC ALK Break Apart Probe 400 μl
C-3056-400 ZytoDot 2C SPEC FGFR2/CEN 10 Probe 400 μl
C-3057-400 ZytoDot 2C SPEC MET/CEN 7 Probe 400 μl
C-3058-400 ZytoDot 2C SPEC ERG Break Apart Probe 400 μl
C-3059-400 ZytoDot 2C SPEC EML4 Break Apart Probe 400 μl
C-3062-400 ZytoDot 2C SPEC CDK4/CEN 12 Break Apart Probe 400 μl
C-3063-100 ZytoDot 2C SPEC ROS1 Break Apart Probe 100 μl
C-3063-400 ZytoDot 2C SPEC ROS1 Break Apart Probe 400 μl
C-3064-100 ZytoDot 2C SPEC RET Break Apart Probe 100 μl
C-3064-400 ZytoDot 2C SPEC RET Break Apart Probe 400 μl
C-3065-100 ZytoDot 2C SPEC FOXO1 Break Apart Probe 100 μl
C-3066-400 ZytoDot 2C SPEC MYC Break Apart Probe 400 μl
C-3067-400 ZytoDot 2C SPEC CDKN2A/CEN 9 Probe 400 μl
C-3068-100 ZytoDot 2C SPEC ERBB2/D17S122 Probe 100 μl
C-3071-100 ZytoDot 2C SPEC IGH Break Apart Probe Probe 100 μl
C-3072-100 ZytoDot 2C SPEC MALT1 Break Apart Probe 100 μl
C-3073-100 ZytoDot 2C SPEC BCL2 Break Apart Probe 100 μl
C-3074-100 ZytoDot 2C SPEC BCL6 Break Apart Probe 100 μl
C-3075-100 ZytoDot 2C SPEC CCND1 Break Apart Probe 100 μl
C-3076-10 ZytoDot 2C Glioma 1p/19q Probe Set
C-3076-40 ZytoDot 2C Glioma 1p/19q Probe Set
C-3077-100 ZytoDot 2C SPEC USP6 Break Apart Probe 100 μl
C-3078-100 ZytoDot2C SPEC NTRK1 Break Apart Probe 100 μl
CAT.# Presentation Vol/Qty
C-3003-40 ZytoDot SPEC HER2 Probe Kit 40
C-3004-40 ZytoDot Pretreatment Kit 40
C-3005-40 ZytoDot CISH Polymer Detection Kit 40
C-3018-40 ZytoDot CISH Implementation Kit 40
C-3022-10 ZytoDot 2C SPEC HER2/CEN 17 Probe Kit 10
C-3022-40 ZytoDot 2C SPEC HER2/CEN 17 Probe Kit 40
C-3028-40 ZytoDot 2C CISH Polymer Detection Kit 40
C-3044-10 ZytoDot 2C CISH Implementation Kit 10
C-3044-40 ZytoDot 2C CISH Implementation Kit 40
CAT.# Product Name
C-3005-10/-40 ZytoDot CISH Polymer Detection Kit
C-3018-10/-40 ZytoDot CISH Implementation Kit
C-3028-40 ZytoDot 2C CISH Polymer Detection Kit
C-3044-10/-40 ZytoDot 2C CISH Implementation Kit
C-3004-40 ZytoDot Pretreatment Kit
ES-0001-4/-8/-50/-1000 Pepsin Solution
PT-0002-500 Heat Pretreatment Solution EDTA
AB-0001-04/-30 Mouse-anti-DIG
AB-0002-4 Anti-Mouse-HRP-Polymer
AB-0013-4 HRP/AP-Polymer-Mix
AB-0014-4 Anti-DIG/DNP-Mix
BS-0001-4 Blocking Solution
C-3011-40 ZytoDot Wash Buffer Set
C-3015-100 ZytoDot DAB Solution Set
C-3038-100 ZytoDot AP-Red Solution Set
C-3039-100 ZytoDot HRP-Green Solution Set
CS-0001-20 Mayer’s Hematoxylin Solution
CS-0002-20 Nuclear Blue Solution
E-4007-2 ERBB2 Control Slide Set
MT-0004-4 Mounting Solution (Alcoholic)
WB-0001-500 Wash Buffer SSC
WB-0004-1000 PBS/Tween
WB-0005-50 20x Wash Buffer TBS