Compact System for Molecular Pathology
The TintoDetector ImmunoDNA System allows users to perform Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) and In-situ Hybridization (ISH) in one easy to use open platform.
TintoDetector ImmunoDNA System Overviews and Features
The Bio SB TintoDetector ImmunoDNA System is a compact molecular pathology system which allows for the full implementation of immunohistochemical (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) and in-situ hybridization (ISH) protocols. Ideal for use in diagnostic or research laboratories looking for a cost-effective, semi-automated and open platform.
The Bio SB TintoDetector ImmunoDNA System consists of a durable stainless steel housing, eight solution dishes, incubator unit (for reagent incubation, nucleic acid denaturation, probe hybridization and stringency washes), and 30-well reagent holders. With a compact footprint, ease of use and versatility, the TintoDetector offers flexible protocol implementation at a competitive price.
- Eight staining dishes for reagents and buffers.
- Built in incubator for ISH protocols that reaches 110 degrees celsius, stores 3 incubator presets.
- Stainless steel construction that is chromogen and xylene resistant.
- Small bench footprint with low power requirements.
- Supports ICC, IHC, CISH, and FISH protocols. Open system
TintoDetector Lab Applications
TintoDetector Components
The TintoDetector Incubator is capable of reaching temperatures up to 110 degrees celsius, and is capable of storing 3 temperature presets. The incubator is used to apply varying temperatures typicaly used inCISH and FISH protocols that involve the denaturing of nucleic acids, probe hybridization and other steps related to CISH & FISH implementation.
The TintoDetector Slide Holder is an extremly durable capillary gap slide holder that is capable of holding 20 capillary gap slides, and easily fits into the TintoDetector Incubator. The TintoDetector slide holder can be used for all IHC, ICC, IF, CISH and FISH applications.
When using the TintoDetector slide Holder, be sure the following procedures are followed:
- Slides are paired face to face.
- if a single slide or odd number must be used, pair with a blank slide.
- Insert slides so that portion of slide with white triangles faces downward when unit is held.
- Unsure that Prone On Plus slides are used.
- Ensure all bottom edges of slides are aligned to ensure proper capillary gap action.
The TintoDetector 30 well reagent holder allows for the application of up to 200 microliters of reagent to a paired set of slides. The 30-well reagent holders can be used to apply any reagent used in IHC, ICC, IF, CISH and FISH Protocols.
| Catalog No. | Component |
| BSB 7000 | TintoDetector System |
| BSB 7002 | TintoDetector Incubator |
| BSB 7004 | TintoDetector 30-Well Reagent Holder |
| BSB 7036 | TintoDetector Absorbent Pads |
| Catalog No. | Component |
| BSB 7003 | TintoDetector Slide Holder |
| BSB 7006 | TintoDetector Cap Gap Plus Slides |
| BSB 7007 | TintoDetector Cap Gap Slides |
| BSB 7009 | Plastic Staining Dish |
TintoDetector Wash Procedure
Step 1 – Load Slides
Load slides in TintoDetector Holder, face to face and properly ordered.
Step 2 – Apply Reagents to Reagent Holder
Apply reagent to TintoDetector 30-well Reagent Holder. Each reagent well can hold about 200 μl of reagent.
Step 3 – Draw Reagents into Slides
Place TintoDetector slide holder over 30-well reagent holder, ensuring that reagents line up with slides. Press slide holder gently against reagent holder. Capillary gap action will draw reagent. Transfer TintoDetector Slide Holder to Incubator.
Step 4 – Incubate
Incubate Slide Holder using the TintoDetector Incubator.
Step 5 – Rinse
After the reagent incubation, eliminate the used reagent into an absorbent pad by gently pressing the slides on the absorbent pad to drain the liquid. Draw washing buffer into the capillary space and repeat the washing process 3 to 5 times. After washing proceed to draw the next step reagent before another incubation.
