Ancillaries

For IVD Use
For Research Use Only
Safety Data Sheet
Intended UseFor Mohs In Vitro Diagnostic Use
Summary and Explanation

The Mohs ImmunoDigestor is used for the enzymatic digestion of frozen tissues prior to immunohistochemical (IHC) procedures.

The enzymatic digestion of frozen tissues fixed with acetone or NBF 10%, improves accessibility of antibodies to epitopes within tissue sections.  In the case of IHC, proteolytic digestion exposes certain epitopes which have been masked during fixation, especially with NBF10%.

PresentationThe Mohs ImmunoDigestor is provided in liquid form ready-to-use.  It contains optimized Proteinase K, a buffer solution, detergents, and sodium azide anti-microbial.
Availability
CAT.#DILUTIONVOL
BSB 0324Ready-To-Use15.0 ml
BSB 0325Ready-To-Use50.0 ml
BSB 0326Ready-To-Use100.0 ml

Recommended Protocol

  1. For Frozen Mohs sections fixed for 2 min. with Acetone or 10% NBF 10%, incubate slides with ImmunoDNA Digestor for 1 minute at 37°C or at room temperature.
  2. Rinse slides briefly in distilled or deionized water (10-20 seconds).
  3. Proceed with Mohs IHC protocol.

Abbreviated Mohs Immunohistochemical Protocol

StepMohs PolyDetector HRP Green or DAB 5 min Protocol Mohs PolyDetector HRP Green or DAB 10 min Protocol
Peroxidase/AP Blocker0.5 min.0.5 min.
Primary Antibody2 min4 min.
1st Step Detection1 min3 min.
Substrate-Chromogen1 min2 min.
Counterstain / Coverslip0.5 min0.5 min.