| Intended Use | For Mohs In Vitro Diagnostic Use | |||||||||||||||
| Summary and Explanation | The Mohs ImmunoDigestor is used for the enzymatic digestion of frozen tissues prior to immunohistochemical (IHC) procedures. The enzymatic digestion of frozen tissues fixed with acetone or NBF 10%, improves accessibility of antibodies to epitopes within tissue sections. In the case of IHC, proteolytic digestion exposes certain epitopes which have been masked during fixation, especially with NBF10%. | |||||||||||||||
| Presentation | The Mohs ImmunoDigestor is provided in liquid form ready-to-use. It contains optimized Proteinase K, a buffer solution, detergents, and sodium azide anti-microbial. | |||||||||||||||
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Recommended Protocol
- For Frozen Mohs sections fixed for 2 min. with Acetone or 10% NBF 10%, incubate slides with ImmunoDNA Digestor for 1 minute at 37°C or at room temperature.
- Rinse slides briefly in distilled or deionized water (10-20 seconds).
- Proceed with Mohs IHC protocol.
Abbreviated Mohs Immunohistochemical Protocol
| Step | Mohs PolyDetector HRP Green or DAB 5 min Protocol | Mohs PolyDetector HRP Green or DAB 10 min Protocol |
| Peroxidase/AP Blocker | 0.5 min. | 0.5 min. |
| Primary Antibody | 2 min | 4 min. |
| 1st Step Detection | 1 min | 3 min. |
| Substrate-Chromogen | 1 min | 2 min. |
| Counterstain / Coverslip | 0.5 min | 0.5 min. |
