cd34 anti-cd34 antibody immunohistochemistry ihc

CD34 Antibody Immunohistochemistry on an FFPE Dermatofibrosarcoma Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and Explanation

CD34 functions as a cell-cell adhesion factor and cell-surface glycoprotein. It may also mediate the attachment of stem cells to bone marrow extracellular matrixes or directly to stromal cells. Cells expressing CD34 are normally found in the umbilical cord and bone marrow as hematopoletic cells, and in vascular endothelium. In addition to stem cell recognition, CD34 is expressed by vascular endothelium; it appears that proliferating endothelial cells express this molecule in greater amounts than resting cells. In comparison to factor VIII R Antigen, CD34 stains are stronger and appear to be more sensitive in nature.

In tumors, CD34 antibody expression is found in Alveolar Soft Part Sarcoma, pre B-ALL (positive in 75%), AML(40%), AMLM7 (most), Dermatofibrosarcoma Protuberans, Gastrointestinal Stromal Tumors, Giant Cell Fibroblastoma, Granulocytic Sarcoma, Kaposi’s Sarcoma, Liposarcoma, Malignant Fibrous Histiocytoma, Malignant Peripheral Nerve Sheath tumors, Mengingeal Hemangiopericytomas, Meningiomas, Neurofibromas, Schwannomas, and Papillary Thyroid Carcinoma. A negative CD34 may exclude Ewing’s Sarcoma/PNET, Myofibrosarcoma of the breast, and Inflammatory Myofibroblastic tumors of the stomach.

Antibody TypeRabbit MonoclonalCloneEP88
IsotypeIgGReactivityParaffin, Frozen
LocalizationMembranousControlTonsil, Placenta, Appendix
PresentationCD34 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 6485PredilutedReady-To-Use3.0 ml
BSB 6486PredilutedReady-To-Use7.0 ml
BSB 6487PredilutedReady-To-Use15.0 ml
BSB 6488Concentrated1:50-1:2000.1 ml
BSB 6489Concentrated1:50-1:2000.5 ml
BSB 6490Concentrated1:50-1:2001.0 ml
BSB 6491Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Synonyms: CD34, sc74499, sc-74499, 74499, anti-CD34, anti CD34, CD34 stem cell marker, anti-CD34 stem cell marker, anti CD34 stem cell marker, stem cell markers, anti-stem cell markers, anti stem cell markers, CD34 antigen, CD34 molecule, Hematopoietic progenitor cell antigen CD34, AU040960, cluster designation 34, My 10, L selectin ligand, Mucosialin, gp105 120, gp105 120 s, tem cell progenitor progenitor cell, anti-cd34, anti cd34