BOB-1 BOB.1 BOB1 BOB 1 anti-BOB-1 anti-BOB.1 anti-BOB1 anti-BOB 1 antibody immunohistochemistry ihc

BOB-1 Antibody Immunohistochemistry on an FFPE Tonsil Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

The BOB-1 protein is a co-activator that interacts with OCT-1 and/or OCT-2 transcription factors, and is critical in germinal center formation and immunoglobulin production. The strongest expression of BOB-1 is found in the germinal center, mantle-zone B cells, and plasma cells. Because BOB.1/OBF.1 are germinal center derived, L&H cells in Nodular Lymphocyte Predominant Hodgkin’s Lymphoma are consistently immunoreactive for BOB-1. Conversely, the Hodgkin/Reed-Sternberg cells in classical Hodgkin’s Lymphoma either do not express both or express only one of the two proteins or neither.

In Diffuse Large B-cell Lymphomas, the highest expression levels for BOB-1 antibody are reported in Follicular Center Lymphomas, Diffuse Large B-cell Lymphomas, and Burkitt Lymphomas. B-CLL, MALT-type, and Mantle Cell Lymphomas score negative or display a heterogenous/weaker activity. The strong nuclear expression of BOB-1 antibody and Oct-2 by Germinal Center Derived Lymphomas makes these antibodies a novel class of broad spectrum B-lineage immunohistochemical markers in the differential diagnosis of Lymphomas, specifically between Primary Mediastinal B-cell Lymphoma from classical Hodgkin’s Disease .

Antibody Type Rabbit Monoclonal Clone RBT-BOB1
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Nuclear Control Tonsil, Lymph Node
Presentation BOB-1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3700-3 Prediluted Ready-To-Use 3.0 ml
BSB 3700-7 Prediluted Ready-To-Use 7.0 ml
BSB 3700-15 Prediluted Ready-To-Use 15.0 ml
BSB 3700-01 Concentrated 1:250-1:1000 0.1 ml
BSB 3700-05 Concentrated 1:250-1:1000 0.5 ml
BSB 3700-1 Concentrated 1:250-1:1000 1.0 ml
BSB 3700-CS Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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