VisionArray Analysis Package
The VisionArray Analysis Package stands for a simple visualization and a quick analysis of the VisionArray Chip data. The analysis of a chip and the report of the results can be achieved in just a few minutes.
VisionArray HPV Chip – Fast and Reliable Genotyping
The human papillomavirus (HPV) has been conclusively identified as the major risk factor for cervical cancer. It is the third most common cancer in women worldwide, with an estimated number of 530,000 new cases and 280,000 deaths each year. Currently more than 150 HPV types are well characterized which are classified as high-risk and low-risk types depending on the risk of progression from mild dysplasia to cancer.
The VisionArray HPV Chip is intended to be used with the VisionArray Analysis Package for the qualitative detection and genotyping of PCR amplificates of 41 clinically relevant HPV genotypes that have been produced with the help of the VisionArrayHPV Primer Kit and the VisionArray Detection Kit.
Importance of HPV Testing
The human papillomavirus (HPV) has been conclusively identified as the major risk factor for cervical cancer. It is the third most common cancer in women worldwide, with an estimated number of 530,000 new cases and 280,000 deaths each year. Over the last years the relevance of HPV in the history of oropharyngeal cancers has become more and more important which is indicated by a dramatically risen number of cancers of the oral cavity and pharynx linked to HPV.
Cervical Cancer Progression
Initial HPV infection requires virion integration into the basal epithelial cells. Whereas most HPV infections are transient and are cleared by the immune system, infection with HPV high risk types may lead to cancer when infection persists.
VisionArray HPV Chip – Fast and Reliable Genotyping
The VisionArray HPV Chip 1.0 is intended to be used with the VisionArray Analysis Package for the qualitative detection and genotyping of PCR amplificates of 41 clinically relevant HPV genotypes.
1. Sample Collection
For the detection of HPV genotypes with the VisionArray ® HPV system the following raw material can be used for DNA extraction:
- Formalin-fixed, paraffin-embedded (FFPE) tissue samples
- Cervical swab/brush specimen
- Liquid based cytology specimen (ThinPrep®)
2. Detection & Differention of 41 HPV Types
Step 1: Amplification and Labeling in a PCR
Biotinylated primers are used to amplify and label different sections of the L1 region of the HPV genome. The human HLA-DQA1 gene is also amplified and serves as a PCR positive control and as a genomic control.
Step 2: Hybridization on the Glass Chip
After amplification, the biotinylated sequences hybridize to complementary DNA capture sequences on the glass chip.
Step 3: Detection and Visualization
Specifically bound and biotinylated sequences are visualized by secondary marking with a streptavidinperoxidase conjugate and a staining with tetramethylbenzidine. After color development, evaluation is performed using the VisionArray Analyzer Software.
3. Workflow Schedule
This is a condensed protocol for the VisionArray method and should not replace the instruction for use!
Quick & easy 1 hour protocol · Automated user-friendly evaluation within a few minutes
HPV Tests in Comparison
VisionArray – At a Glance
- Simultaneous genotyping of 41 different HPV types – all certified for in vitro diagnostic use
- All capture sequences and positive controls are set up on the VisionArray Chip as duplicates
- High sensitivity and specificity
- Quick & easy 1 hour protocol
- Automated evaluation using the VisionArray Analyzer Software – simple visualization & quick analysis in just a few minutes
VisionArray Analysis Package MultiScan
Product Manual Short Protocol The VisionArray Analysis Package MultiScan stands for a simple visualization and quick analysis of up to six VisionArray Chips in a single run. The VisionArray Analyzer Software MultiScan can thereby analyze HPV and MYCO specific Chips simultaneously. Only a few minutes will pass from scan to report. CAT.# DESCRIPTION QTY BSB 4070-1 VisionArray Analysis…
VisionArray Cytology DNA Extraction Kit
Product Brochure Product Manual The VisionArray Cytology DNA Extraction Kit is designed to be used for the rapid isolation of genomic DNA from liquid-based cytology samples in order to achieve small-scale preparations of highly pure genomic DNA. The purified DNA can be used directly for PCR with the VisionArray HPV Primer Kit, the VisionArray PreCise Taq DNA Polymerase,…
VisionArray Detection Kit
The VisionArray Detection Kit contains all reagents that are required for the simple and fast hybridization and detection of PCR products on VisionArray Chips, produced with the VisionArray Primer Kit, on the corresponding VisionArray Chip.
VisionArray FFPE DNA Extraction Kit
Product Brochure Product Manual The VisionArray FFPE DNA Extraction Kit is designed to be used for the isolation of genomic DNA from formalin-fixed, paraffin-embeded (FFPE) specimens. The professional use of this purification system optimized for FFPE samples results in highly purified nucleic acid samples. The purified DNA can be used directly for PCR with the VisionArray HPV…
VisionArray HPV Chip 1.0 – Detection of 41 HPV Types
The VisionArray HPV Chip 1.0 is intended to be used with the VisionArray Analysis Package for the qualitative detection and genotyping of PCR amplificates of 41 clinically relevant Human Papilloma Virus (HPV) genotypes that have been produced with the help of the VisionArray HPV Primer Kit and the VisionArray Detection Kit.
VisionArray HPV PreCise Master Mix
Product Manual Short Protocol The VisionArray HPV PreCise Master Mix is a ready to use PCR Master Mix for amplifying the genomes of HPV types including but not limited to those detected by the VisionArray HPV Chips. The VisionArray PreCise Master Mix contains the VisionArray HPV Primer Mix 2.0, dNTP/dUTP Solution, the VisionArray PreCise Taq DNA Polymerase, the PCR-Buffer, MgCl2,…
VisionArray HPV Primer Kit 2.0
The VisionArray HPV Primer Kit is intended to be used for amplification and biotinylation of specific sections of the L1 region of the Human Papilloma Virus (HPV) genomes by polymerase chain reaction (PCR). The hybridization and detection of the amplified sequences is performed subsequently using the VisionArray HPV Chip and the VisionArray Detection Kit.
VisionArray PreCise Taq DNA Polymerase
Product Manual Short Protocol The VisionArray PreCise Taq DNA Polymerase is a high quality heat stable Taq polymerase intended to be used for the specific amplification of a wide range of DNA fragments. The enzyme has hot-start capabilities, reduces false positives, amplifies low-level target sequences, and promotes amplification of a variety of templates. Further on, neccessary…
VisionArray Uracil-DNA Glycosylase
Product Manual Short Protocol The VisionArray Uracil-DNA (UDG) Glycosylase is an E. coli derived enzyme intended to be used to prevent carry-over contaminations from previous reactions. This is based on the principle of substituting dUTPs for dTTPs during PCR. Therefore the PCR amplificate can be “discriminated” from the initital DNA strand. Incubating the PCR mixture with the UDG…