Direct immunofluorescence uses a single, primary antibody, chemically linked to a fluorophore or fluorochrome. The primary antibody recognizes the target molecule (antigen) and binds to a specific region called the epitope. The attached fluorophore can be detected via fluorescent microscopy, which, depending on the fluorophore used, will emit a specific wavelength of light once excited. Direct immunofluorescence, although somewhat less common, has notable advantages over the indirect procedure. The direct detection of the antigen by a primary antibody directly labeled with a fluorochrome reduces the number of steps in the procedure, saving time and reducing non-specific background signal.
- High Affinity Purified Antibodies Conjugated to FITC
- Highly Sensitive and Specific
- Low Background
- Validated for Transplantation and Autoimmunity Studies