Compact System for Immunocytochemistry, Immunofluorescence and Immunohistochemistry

Perform Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) and In-situ Hybridization (ISH) in one easy to use open platform.

A demonstration of the Fast Mohs technique on our Bio SB TintoDetector ImmunoDNA manual staining system.
Featuring our selection of TintoFast antibodies, and detection systems for fast IHC cancer detection after Mohs surgery.

Overviews and Features

The Bio SB TintoDetector Mini Immuno System is a capillary gap based system which allows for full implementation of Immunocytochemistry (ICC), Immunofluorescence (IF) and Immunohistochemistry (IHC) protocols in your diagnostic laboratory. The Bio SB TintoDetector Mini is intended for laboratories which require flexibility in their diagnostic needs, yet may not require high volume slide output offered by larger systems, such as the full-size Bio SB TintoDetector or the automated Bio SB TintoStainer.

The Bio SB TintoDetector Mini Immuno System is an open system that allows for the use of any detection system or reagent. The TintoDetector Mini paired with accessories, like the Bio SB TintoRetriever Pressure Cooker, allows laboratories to implement a wide variety of lab protocols at a low cost.

  • Four Staining Dishes for reagents and buffers.
  • Five 30-Well Reagent Holders
  • Stainless steel construction that is chromogen and xylene resistant.
  • Small bench footprint.
  • Supports ICC, IHC, and IF protocols. Open system.

TintoDetector Mini Components

The TintoDetector Mini ImmunoDNA System is constructed out of stainless steel, that is chromogen and xylene resistant, for sustained durability. It also provides a small footprint for easy installation or relocation.

The TintoDetector Slide Holder is an extremly durable capillary gap slide holder that is capable of holding 20 capillary gap slides. The TintoDetector slide holder can be used for all IHC, ICC, IF applications.

When using the TintoDetector slide Holder, be sure the following procedures are followed:

  • Slides are paired face to face.
  • if a single slide or odd number must be used, pair with a blank slide.
  • Insert slides so that portion of slide with white triangles faces downward when unit is held.
  • Unsure that Prone On Plus slides are used.
  • Ensure all bottom edges of slides are aligned to ensure proper capillary gap action.


The TintoDetector 30 well reagent holder allows for the application of up to 200 microliters of reagent to a paired set of slides. The 30-well reagent holders can be used to apply any reagent used in IHC, ICC, IF Protocols.

All TintoDetector staining dishes are capable of holding solutions such as buffers, or special stains for use in the IHC protocols. All staining dishes are capable of holding 200 mL of solution.

Catalog No.Component
BSB 7085TintoDetector Mini Immuno System
BSB 7004TintoDetector 30-Well Reagent Holder
BSB 7036 TintoDetector Absorbent Pads
Catalog No.Component
BSB 7003TintoDetector Slide Holder
BSB 7006TintoDetector Cap Gap Plus Slides
BSB 7009Plastic Staining Dish

TintoDetector Mini Wash Procedure

Step 1 – Load Slides

Load slides in TintoDetector Holder, face to face and properly ordered.

Step 2 – Apply Reagents to Reagent Holder

Apply reagent to TintoDetector 30-well Reagent Holder. Each reagent well can hold about 200 μl of reagent.

Step 3 – Draw Reagents into Slides

Place TintoDetector slide holder over 30-well reagent holder at an angle, ensuring that reagents line up with slides. Press slide holder against reagent holder. Capillary gap action will draw reagent. Transfer TintoDetector Slide Holder to an empty staining dish used as a support.

Step 4 – Incubate

Incubate Slide Holder using an empty staining dish.

Step 5 – Rinse

After the reagent incubation, eliminate the used reagent into an absorbent pad by gently pressing the slides on the absorbent pad to drain the liquid. Draw washing buffer into the capillary space and repeat the washing process 3 to 5 times. After washing proceed to draw the next step reagent before another incubation.