Surfactant Protein D Antibody Immunohistochemistry on an FFPE Lung Squamous Cell Carcinoma Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation|
Surfactant protein D, also known as SFTPD or SP-D, is a protein encoded by the SFTPD gene. Pulmonary surfactants are essential to proper respiratory structure and function, consisting of ~90% lipids (mostly phospholipids) and 8–10% surfactant-associated proteins. SP-D is a pattern-recognition molecule in the collectin (collagen-containing C-type lectin) family. In the lungs, it is an opsonin that can agglutinate a range of microbes and enhance their clearance via phagocytosis and super-oxidative burst. It can interfere with allergen–IgE interaction and suppress basophil and mast cell activation.
Other surfactant proteins like SP-B and SP-C showed strong immunohistochemical expression in Lung Hyperplasias and Adenomas, suggesting that SP-B and SP-C are related to lung tumorigenesis. SP-D is likely an innate immune surveillance molecule against tumor development. SP-D suppresses Lung Cancer progression via interference with epidermal growth factor signaling, and a truncated recombinant human SP-D may induce apoptosis in Pancreatic Adenocarcinoma via Fas-mediated pathway in a p53-independent manner. Studies Have found low expression of Surfactant protein D antibody expression in Lung, Gastric, and Breast cancers and high expression in different stages and grades of Ovarian cancer. Surfactant protein D antibody expression could be associated with a favorable prognosis in Lung Cancer but unfavorable in non-pulmonary sites such as Gastric, Breast, and Ovarian cancers. SP-D detected in circulating tumor cells of Ovarian Cancer patients suggests SP-D can also be used as a potential biomarker.
The purified Spike protein of SARS-CoV-2 bound to Vero but not 293T cells and was itself recognized by SP-D, in the lung alveoli. It suggests that SARS-CoV interacts with innate immune mechanisms in the lung through its S-protein and regulates pulmonary inflammation.
|Antibody Type||Mouse Monoclonal||Clone||BSB-162|
|Localization||Cytoplasmic, Membranous||Control||Placenta, Lung, Pancreas, Adrenal Gland, Lung Squamous Cell Carcinoma, Lung Adenocarcinoma, Gastric GIST|
|Presentation||Surfactant Protein is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|