IHC of ROS1 on an FFPE Non-Small Cell Lung Cancer Tissue

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Research Use Only
Safety Data Sheet

SDS

Control Slides
Intended Use For In Vitro Diagnostic Use
Summary and Explanation Repressor of Silencing 1 (ROS1) is a receptor tyrosine kinase that undergoes genetic rearrangements in various human cancers and in humans is encoded by the ROS1 gene. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity with structural similarity to the anaplastic lymphoma kinase (ALK) protein. The protein may function as a growth or differentiation factor receptor. ROS1 expression is limited in normal tissues to occasional staining cerebellum, stomach, small intestine, colon and kidney.

Gene rearrangements involving the ROS1 gene were first detected in glioblastoma tumors and cell lines.  ROS1 fusion partners include CD74, SLC34A2 and SDC4, leading to oncogenic transformation. ROS1 rearrangement was identified in a cell line derived from a lung adenocarcinoma patient and multiple studies have demonstrated its incidence in lung cancers. While ROS1 is undetectable in the normal lung, studies have described ROS1 rearrangements in 1-2% of NSCLC by FISH. Recent reports have demonstrated strong correlation between ROS1 IHC with FISH positivity. ROS1 fusions have been detected in multiple other tumors, including glioblastoma, non-small cell lung cancer (NSCLC), cholangiocarcinoma, ovarian cancer, gastric adenocarcinoma, colorectal cancer, inflammatory myofibroblastic tumor, angiosarcoma, and epithelioid hemangioendothelioma.

Antibody Type Rabbit Monoclonal Clone EP282
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Placenta, Lung, SiHa Cells, NSCL ROS1 +
Presentation ROS-1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3623 Prediluted Ready-To-Use 3.0 ml
BSB 3624 Prediluted Ready-To-Use 7.0 ml
BSB 3625 Prediluted Ready-To-Use 15.0 ml
BSB 3626 Concentrated 1:50-1:200 0.1 ml
BSB 3627 Concentrated 1:50-1:200 0.5 ml
BSB 3628 Concentrated 1:50-1:200 1.0 ml
BSB 3629 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.