IHC of PHOX2B on an FFPE Neuroblastoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Paired-like homeobox 2b (PHOX2B), also known as neuroblastoma Phox (NBPhox), is a protein that in humans is encoded by the PHOX2B gene located on chromosome 4.  It is expressed exclusively in the nervous system, in most neurons that control the viscera (cardiovascular, digestive and respiratory systems). It is also required for neuron differentiation. Mutations in human PHOX2B cause a rare disease of the autonomic nervous system (dysautonomia): congenital central hypoventilation syndrome (associated with respiratory arrests during sleep and, occasionally, wakefulness), Hirschsprung’s disease (partial agenesis of the enteric nervous system), ROHHAD, and tumors of the sympathetic ganglia.

PHOX2 gene over-expression in Neuroblastoma (NB) tumors and cell lines suggests these genes may be widely involved in Neuroblastoma development through either a direct mechanism of up-regulation or a failure in maintaining proper transcript levels after embryonic development. The PHOX2B expression has been observed in all peripheral neuroblastic tumors, paragangliomas, and pheochromocytomas tested but in no other pediatric tumors among the 388 cases studied by expression microarray and the 109 cases studied by immunohistochemical analysis. PHOX2B and CD57 have been found to be useful markers of Neuroblastoma. PHOX2B is specific for Neuroblastoma in its differential diagnosis with other small round cell tumors, and its nuclear staining may be helpful for accurate bone marrow tumor quantification.

Antibody Type Rabbit Monoclonal Clone EP312
Isotype IgG Reactivity Paraffin, Frozen
Localization Nuclear Control Adrenal, Neuroblastoma
Presentation PHOX2B is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3609 Prediluted Ready-To-Use 3.0 ml
BSB 3610 Prediluted Ready-To-Use 7.0 ml
BSB 3611 Prediluted Ready-To-Use 15.0 ml
BSB 3612 Concentrated 1:25-1:100 0.1 ml
BSB 3613 Concentrated 1:25-1:100 0.5 ml
BSB 3614 Concentrated 1:25-1:100 1.0 ml
BSB 3615 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.