IHC of PD-1 on an FFPE Tonsil Tissue

PD-1 Antibody Immunohistochemistry on an FFPE Tonsil Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and Explanation

Programmed Death 1 (PD-1 or CD279) is a Type I membrane protein comprised of 268 amino acids. PD-1 is a member of the extended CD28/CTLA-4 family of T-cell regulators. PD-1 is expressed on the surface of activated T-cells, B-cells, and macrophages. In comparison to CTLA-4, PD-1 more broadly negatively regulates immune responses.

New data suggests that expression of PD-L1 on tumor cells inhibits anti-tumor activity through engagement f PD-1 on effector T-cells. Expression of PD-L1 on tumors is correlated with reduced survival in esophageal, pancreatic and other types of cancers, highlighting the relevance of exploring the PD-1 pathway as a target for immunotherapy. Studies have found that PD-1antibody is expressed on most T-cells and a small subset of B-cells in the light zone of germinal centers, but not elsewhere in the tonsil. On that basis, it was postulated that PD-1 may play a role in the process of clonal selection of centrocytes, which occurs in this subanatomic site in germinal centers. PD-1 antibody is a new marker of Angioimmunoblastic Lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and bcl-6, PD-1 is expressed by few B-cells, so it may be a more specific and useful diagnostic marker in Angioimmunoblastic Lymphoma. It also seems to stain a greater percentage of CD3-positive neoplastic cells in Angioimmunoblastic Lymphoma than either CD10 or bcl-6.

Antibody TypeMouse MonoclonalCloneNAT-105
IsotypeIgG1ReactivityParaffin, Frozen
LocalizationCytoplasmic, MembranousControlTonsil, Lymph Node
PresentationPD-1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 6212PredilutedReady-To-Use3.0 ml
BSB 6213PredilutedReady-To-Use7.0 ml
BSB 6214PredilutedReady-To-Use15.0 ml
BSB 6215Concentrated1:100-1:5000.1 ml
BSB 6216Concentrated1:100-1:5000.5 ml
BSB 6217Concentrated1:100-1:5001.0 ml
BSB 6218Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Synonyms: Pdcd1, Pdcd 1, pd-1, ant-pd1, anti-pd-1, ant-pd1, anti pd-1, Ly101, mPD 1, pd1, PD 1, Pdc1, programmed cell death 1, programmed cell death protein 1, protein PD 1, RMP114, RMP1 14