IHC of Kappa on a FFPE Kidney Tissue
IF of Kappa on a Frozen Colon Tissue (IF with FluoroMounter DAPI)
For In Vitro Diagnostic Use.
This antibody is intended for use in Immunohistochemical (IHC) and Immunofluorescence (IF) applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.
|Summary and Explanation||
Kappa detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, Kappa exhibits strong staining of kappa-positive plasma cells and cells that have absorbed exogenous immunoglobulin.
When studying B-cell neoplasms, the determination of light-chain ratios remains the centerpiece. This is sound reasoning because most B-cell Lymphomas express either Kappa or Lambda light chains, whereas reactive proliferations display a mixture of Kappa and Lambda-positive cells. If only a single light-chain type is detected, a lympho-proliferative disorder is very likely. Monoclonality is determined by a Kappa-Lambda ratio greater than or equal to 3:1, a Lambda-Kappa ratio greater than or equal to 2:1, or a monoclonal population of 75% or more of the total population.
In IgG-dominant immune complex-mediated glomerulonephritis, there are multiple pathological findings that strongly suggest the diagnosis of Lupus Nephritis including immunofluorescence staining for IgG, IgM, IgA, Kappa or Lambda, C3 and C1.
|Antibody Type||Rabbit Polyclonal||Clone||Polyclonal|
|Localization||Cytoplasmic||Control||Tonsil, Lymph Node|
|Presentation||Kappa is a purified immunoglobulin fraction of rabbit antiserum that is filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|
*Ready-to-Use, for IHC only!