IHC of INI-1 on an FFPE Ewing’s Sarcoma Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/ SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1, that differ by the variable inclusion of amino acids, potentially are produced by differential RNA splicing.
The morphology of MRTs can present challenges in differential diagnosis. The overall survival of MRTs relative to its potential mimics (medulloblastoma, supratenorial primitive neuroectodermal tumors (sPNETs)) is quite low, and thus differentiation from these other tumors is desirable. Lack of nuclear labeling by anti-INI-1 is characteristic of MRT. The majority of medulloblastomas and sPNETs are labeled by anti-INI-1. MRTs also originate from the kidney and soft tissues.
|Antibody Type||Mouse Monoclonal||Clone||25|
|Localization||Nuclear||Control||Testis, Brain, Breast, Colon, Kidney, Pituitary, Adrenal, Prostate, Thyroid, Lung, Pancreas, Cervix, Salivary Gland, Astrocytoma, Lymphoblastic Lymphoma, Transitional Cell Carcinoma|
|Presentation||INI-1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|