IFN-a Antibody Immunohistochemistry on an FFPE Papillary Thyroid Carcinoma Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation|
Type I interferons are a large subgroup of interferon proteins that help regulate the immune system by binding to IFN-⍺ receptors. IFN-⍺ (IFN-a) is produced mainly by plasmacytoid dendritic cells and involved in innate immunity against viral infections. Binding of IFN-⍺ to its receptor leads to downstream signalling and expression of numerous different IFN-stimulated genes. These genes encode antiviral proteins that directly inhibit viral replication as well as modulate immune function.
Studies have shown IFN-a strongly modulates innate and adaptive immune responses in the host by enhancing the proliferation, cytotoxicity and IFN-γ secretion of NK cells, as well as acting as a pyrogenic factor by altering the activity of thermosensitive neurons in the hypothalamus which causes fever. It does this by binding to opioid receptors and eliciting the release of prostaglandin-E2. IFN-a can also interact with the μ-opioid receptor to act as an analgesic. Additionally, recent studies have shown that Type 1 IFNs stimulate secretion of IP-10 (CXCL10) which is a critical chemokine to recruit effector T cells to the tumor microenvironment and IP-10 knockout mice exhibit a phenotype with compromised effector T cell generation and trafficking. Type 1 IFNs also induce MHC class 1 upregulation on tumor cells which can enhance antitumor CD8 T cell effector response in the tumor microenvironment.
|Antibody Type||Mouse Monoclonal||Clone||BSB-158|
|Localization||Cytoplasmic, Nuclear||Control||Placenta, Fallopian Tube, Stomach, Prostate, Testis, Transitional Cell Carcinoma, Papillary Thyroid Carcinoma|
|Presentation||IFN-a is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|