IHC of IDH1 R132H on an FFPE Glioma Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||The IDH1 gene on chromosome 2q33.3 encodes for isocitrate dehydrogenase 1 (IDH1), located in the cytoplasm and the peroxisomes. This enzyme catalyzes NADPH production via oxidative decarboxylation of isocitrate to alpha-ketoglutarate in the Krebs citric acid cycle. Studies have shown that IDH1 mutation is an early step in gliomagenesis and has been reported to occur in grades II and III astrocytomas, oligodendrogliomas (OG), oligoastrocytomas (OA), and secondary GBM.
Mutations involving IDH1 occur in a high proportion of diffuse gliomas, with implications on diagnosis. About 90% involve exon 4 at codon 132, replacing amino acid arginine with histidine (R132H). Preliminary studies comparing Immunohistochemistry (IHC) with IDH1-R132H mutation-specific antibodies have shown concordance with DNA sequencing and no cross-reactivity with wild-type IDH1 or other mutant proteins.
|Antibody Type||Mouse Monoclonal||Clone||IHC132|
|Localization||Cytoplasmic||Control||Glioma, Glioblastoma, Astrocytoma with IDH1 R132H Mutation|
|Presentation||ADHL1 R132H is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|