|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition phase of the cell cycle that detremines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1 (an inhibitor of Cyclin D), taggint it for degradation and thus promoting expression of Cyclin A, allowing progression to the S phase. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Apart from the function in cell cycle progression, cyclin E/CDK2 plays a role in the centrosome cycle by phosphorylating nucleophosmin (NPM). NPM is then released from binding to an unduplicated centrosome, thereby triggering duplication. Cyclin E/CDK2 has also been shown to regulate the apoptotic response to DNA damage via phosphorylation of FOXO1.
Overexpression of Cyclin E correlates with tumorigenesis. It is involved in various types of cancers, including breast, colon, bladder, skin, and lung cancer.
|Antibody Type||Rabbit Monoclonal||Clone||EP126|
|Localization||Nuclear||Control||Placenta, Bladder, Colon, Breast Cancer, Ovarian Carcinoma|
|Presentation||Cyclin E1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|