cd3 epsilon anti-cd3 antibody cd3e immunohistochemistry ihc

CD3 Epsilon Antibody Immunohistochemistry on an FFPE Tonsil Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and Explanation

The CD3 antigen is a protein complex composed of three distinct chains (CD3γ, CD3δ and CD3ε) that associate with T-cell receptors and the ζ-chain to generate an activation signal in T-lymphocytes. The TCR, ζ-chain and CD3 molecules together comprise the TCR complex. The CD3γ, CD3δ, and CD3ε chains are highly-related cell surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain. The intracellular tails of the CD3 molecules contain a single conserved motif known as an immunoreceptor tyrosine-based activation motif (or ITAM for short), which is essential for the signaling capacity of the TCR. Phosphorylation of the ITAM on CD3 renders the CD3 chain capable of binding the enzyme ZAP70 (zeta-associated protein), a kinase important in the signaling cascade of the T-cell.

The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency and has also been linked to a susceptibility to type I diabetes in women. CD3 has been considered the best all-around T-cell marker. This antibody reacts with an antigen present in early thymocytes. The positive staining of this marker may represent a sign of early commitment to the T-cell lineage.

Antibody TypeRabbit MonoclonalCloneRBT CD3e
IsotypeIgGReactivityParaffin, Frozen
LocalizationMembranousControlTonsil, Lymph Node
PresentationCD3 Epsilon is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 5141PredilutedReady-To-Use3.0 ml
BSB 5142PredilutedReady-To-Use7.0 ml
BSB 5143PredilutedReady-To-Use15.0 ml
BSB 5144Concentrated1:100-1:5000.1 ml
BSB 5145Concentrated1:100-1:5000.5 ml
BSB 5146Concentrated1:100-1:5001.0 ml
BSB 5147Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Synonyms: CD3E, anti-CD3, anti CD3, anti-CD3E, anti CD3E, CD3 antigen, CD3 monoclonal, anti-CD3 antigen, anti CD3 antigen, anti-CD3 monoclonal, anti CD3 monoclonal, CD3 epsilon, CD3e antigen epsilon polypeptide TiT3 complex, CD3e molecule epsilon CD3 TCR complex, FLJ18683, T cell antigen receptor complex epsilon subunit of T3, T cell surface antigen T3 Leu 4 epsilon chain, T cell surface glycoprotein CD3 epsilon chain, T3E, TCRE