CD3 Epsilon Antibody Immunohistochemistry on an FFPE Tonsil Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation|
The CD3 antigen is a protein complex composed of three distinct chains (CD3γ, CD3δ and CD3ε) that associate with T-cell receptors and the ζ-chain to generate an activation signal in T-lymphocytes. The TCR, ζ-chain and CD3 molecules together comprise the TCR complex. The CD3γ, CD3δ, and CD3ε chains are highly-related cell surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain. The intracellular tails of the CD3 molecules contain a single conserved motif known as an immunoreceptor tyrosine-based activation motif (or ITAM for short), which is essential for the signaling capacity of the TCR. Phosphorylation of the ITAM on CD3 renders the CD3 chain capable of binding the enzyme ZAP70 (zeta-associated protein), a kinase important in the signaling cascade of the T-cell.
The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency and has also been linked to a susceptibility to type I diabetes in women. CD3 has been considered the best all-around T-cell marker. This antibody reacts with an antigen present in early thymocytes. The positive staining of this marker may represent a sign of early commitment to the T-cell lineage.
|Antibody Type||Rabbit Monoclonal||Clone||RBT CD3e|
|Localization||Membranous||Control||Tonsil, Lymph Node|
|Presentation||CD3 Epsilon is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|