brg-1, brg1, anti-brg-1, anti-brg1, smarca-4, anti-smarca-4, smarca4, anti-smarca4

BRG-1 Antibody Immunohistochemistry on an FFPE Ductal Breast Carcinoma Tissue

Intended UseFor In Vitro Diagnostic Use
Summary and Explanation

Brahma-related gene-1 (BRG-1) protein is encoded by the gene SMARCA-4, which is localized on chromosome 19. BRG-1 is the core catalytic ATPase subunit of the SWItch/sucrose non-fermentable (SWI/SNF) complex. SWI/SNFs are a member of the family of ATP-dependent chromatin-remodeling complexes and the function of SWI/SNFs is to facilitate the transcriptional activation or repression of target genes. BRG-1 is essential for DNA repair, differentiation, and organ development. The molecular weight of the BRG-1 subunit is approximately 185 kDa.

There are several studies that suggest the involvement of BRG-1 in different cancer types. It was found that the loss of BRG-1 antibody expression occurs in a portion of tested cancers types, including Breast, Colon, Head/Neck, Ovarian, Liver and Renal Cell Cancer. On the contrary, overexpression of BRG-1 was found in Breast, Colorectal, and Prostate Cancer, as well as Melanoma and Neuroblastoma. An IHC analysis of BRG-1 in Non-Small Cell Lung Cancer (NSCLC) patients revealed that the survival rate of BRG-1 negative patients was 0%, when compared to BRG-1 positive patients, indicating the prognostic value of BRG-1 as a biomarker. Other IHC studies found that the loss of BRG-1 protein expression highly correlates with Small Cell Carcinoma of the Ovary, Hypercalcemic Type (SCCOHT), suggesting BRG-1 as a diagnostic marker for SCCOHT.

Antibody TypeMouse MonoclonalCloneBSB-154
IsotypeIgG1ReactivityParaffin, Frozen
LocalizationNuclearControlColon, Kidney, Prostate, Testis, Transitional Cell Carcinoma, T Cell Lymphoblastic Lymphoma
PresentationBRG-1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB-3714-3PredilutedReady-To-Use3.0 ml
BSB-3714-7PredilutedReady-To-Use7.0 ml
BSB-3714-15PredilutedReady-To-Use15.0 ml
BSB-3714-01Concentrated1:25-1:1000.1 ml
BSB-3714-05Concentrated1:25-1:1000.5 ml
BSB-3714-1Concentrated1:25-1:1001.0 ml
BSB-9038-CSControl Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Synonyms: brg-1, brg1, anti-brg-1, anti-brg1, smarca-4, anti-smarca-4, smarca4, anti-smarca4, brg-1 /smarca4, brg-1/smarca4, /smarca4, smarca4, anti-smarca4, anti Brg-1, anti-Brg 1, anti Brg 1, anti Brg1, anti-SMARCA4, anti SMARCA4, ATP dependent helicase SMARCA4, BAF190, brahma protein like 1, BRM SWI2 related gene 1, global transcription activator homologous sequence, homeotic gene regulator, hSNF2b, mitotic growth and transcription activator, nuclear protein GRB1, SNF2, SNF2 beta, SNF2 like 4, SNF2L4, SNF2LB, sucrose nonfermenting like 4, SWI2, BAF190A, BRG1 associated factor 190A, Protein brahma homolog 1, protein BRG 1, transcription activator BRG1, RTPS2