atrx, anti-atrx, α-thalassemia mental retardation syndrome X-linked

ATRX Antibody Immunohistochemistry on an FFPE Astrocytoma Tissue

Intended UseFor In Vitro Diagnostic Use
Summary and Explanation

α-thalassemia/mental retardation syndrome X-linked (ATRX) gene is located on chromosome Xq21.1. ATRX is involved in many fundamental cellular processes such as transcription, replication, DNA repair and recombination. Germline mutations of ATRX have been found to cause the complex genetic disorder called Alpha-Thalassemia mental retardation syndrome. Somatic mutations, deletions, and altered ATRX expression levels were found to be prevalent in several cancer types. A study reported the loss of ATRX expression was found to be a prognostic marker for chromosome instability in pancreatic neuroendocrine tumors. There is also evidence that highlights the role of ATRX as a biomarker in breast cancer in which ATRX expression was significantly associated with tumor grade.

Mutation/loss of ATRX antibody expression has been described in anaplastic gliomas. A study explored the role of ATRX status in the molecular classification of anaplastic gliomas and its impact on survival. Loss of ATRX antibody expression was detected in 45 % of anaplastic astrocytomas (AA), 27 % of anaplastic oligoastrocytomas (AOA) and 10 % of anaplastic oligodendrogliomas (AO). Survival analysis showed a marked separation of IDH mutant astrocytic tumors into two groups based on ATRX status: tumors with ATRX loss had a significantly better prognosis. Another recent study analyzed the use of ATRX, IDH and 1p/19q codeletion in a series of astrocytomas, oligodendrogliomas, oligoastrocytomas and glioblastomas and presented an algorithm based on stepwise analysis with initial immunohistochemistry for ATRX and IDH1-R132H followed by 1p/19q analysis, then by IDH sequencing, which reduces the number of molecular analyses and has a far better association with patient outcome.

Antibody TypeRabbit MonoclonalCloneRBT-ATRX
IsotypeIgGReactivityParaffin, Frozen
LocalizationNuclearControlBreast, Colon, Fallopian Tube, Brain, Tonsil, Transitional Cell Carcinoma, T Cell Lymphoblastic Lymphoma
PresentationATRX is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB-3711-3PredilutedReady-To-Use3.0 ml
BSB-3711-7PredilutedReady-To-Use7.0 ml
BSB-3711-15PredilutedReady-To-Use15.0 ml
BSB-3711-01Concentrated1:50-1:2000.1 ml
BSB-3711-05Concentrated1:50-1:2000.5 ml
BSB-3711-1Concentrated1:50-1:2001.0 ml
BSB-9022-CSControl Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Synonyms: atrx, anti-atrx, α-thalassemia mental retardation syndrome X-linked