VisionArray HPV Chip – Fast and Reliable Genotyping

The human papillomavirus (HPV) has been conclusively identified as the major risk factor for cervical cancer. It is the third most common cancer in women worldwide, with an estimated number of 530,000 new cases and 280,000 deaths each year. Currently more than 150 HPV types are well characterized which are classified as high-risk and low-risk types depending on the risk of progression from mild dysplasia to cancer.

The VisionArray HPV Chip is intended to be used with the VisionArray Analysis Package for the qualitative detection and genotyping of PCR amplificates of 41 clinically relevant HPV genotypes that have been produced with the help of the VisionArrayHPV Primer Kit and the VisionArray Detection Kit.

Importance of HPV Testing

The human papillomavirus (HPV) has been conclusively identified as the major risk factor for cervical cancer. It is the third most common cancer in women worldwide, with an estimated number of 530,000 new cases and 280,000 deaths each year. Over the last years the relevance of HPV in the history of oropharyngeal cancers has become more and more important which is indicated by a dramatically risen number of cancers of the oral cavity and pharynx linked to HPV.

Cervical Cancer Progression

Initial HPV infection requires virion integration into the basal epithelial cells. Whereas most HPV infections are transient and are cleared by the immune system, infection with HPV high risk types may lead to cancer when infection persists.

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VisionArray HPV Chip – Fast and Reliable Genotyping

The VisionArray HPV Chip 1.0 is intended to be used with the VisionArray Analysis Package for the qualitative detection and genotyping of PCR amplificates of 41 clinically relevant HPV genotypes.

1. Sample Collection

For the detection of HPV genotypes with the VisionArray ® HPV system the following raw material can be used for DNA extraction:

  • Formalin-fixed, paraffin-embedded (FFPE) tissue samples
  • Cervical swab/brush specimen
  • Liquid based cytology specimen (ThinPrep®)

2. Detection & Differention of 41 HPV Types

Step 1: Amplification and Labeling in a PCR

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Biotinylated primers are used to amplify and label different sections of the L1 region of the HPV genome. The human HLA-DQA1 gene is also amplified and serves as a PCR positive control and as a genomic control.

Step 2: Hybridization on the Glass Chip

After amplification, the biotinylated sequences hybridize to complementary DNA capture sequences on the glass chip.

Step 3: Detection and Visualization

Specifically bound and biotinylated sequences are visualized by secondary marking with a streptavidinperoxidase conjugate and a staining with tetramethylbenzidine. After color development, evaluation is performed using the VisionArray Analyzer Software.

3. Workflow Schedule

This is a condensed protocol for the VisionArray method and should not replace the instruction for use!

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Quick & easy 1 hour protocol · Automated user-friendly evaluation within a few minutes

HPV Tests in Comparison

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VisionArray – At a Glance

  • Simultaneous genotyping of 41 different HPV types – all certified for in vitro diagnostic use
  • All capture sequences and positive controls are set up on the VisionArray Chip as duplicates
  • High sensitivity and specificity
  • Quick & easy 1 hour protocol
  • Automated evaluation using the VisionArray Analyzer Software – simple visualization & quick analysis in just a few minutes

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VisionArray HPV Chip 1.0

The VisionArray HPV Chip 1.0 is intended to be used with the VisionArray Analysis Package for the qualitative detection and genotyping of PCR amplificates of 41 clinically relevant Human Papilloma Virus (HPV) genotypes that have been produced with the help of the VisionArray HPV Primer Kit and the VisionArray Detection Kit.

Details

VisionArray HPV Primer Kit

The VisionArray HPV Primer Kit is intended to be used for amplification and biotinylation of specific sections of the L1 region of the Human Papilloma Virus (HPV) genomes by polymerase chain reaction (PCR). The hybridization and detection of the amplified sequences is performed subsequently using the VisionArray HPV Chip and the VisionArray Detection Kit.

Details