Polymerase Chain Reaction (PCR) selectively amplifies discrete segments of DNA. One of the major applications of PCR is to detect and quantify mRNA species through its highly sensitive and specific amplification. The growing abundance of genomic sequence data invites increasingly large-scale genetic analysis, which is gradually proving useful in clinical analysis. Our PCR single primer kits are designed to avoid formation of “primer dimers” during amplification. The PCR primers can be used for regular PCR or Real-time PCR.Our positive control primer is sequence-confirmed and copy number defined, so this can be used as a template for regular PCR or standard curve for real-time PCR. Our single PCR kit can be used with any other company’s SyBr Green kits for Real-time PCR applications.

Bio SB offers complete kits for single, dual and multiplex PCR to meet and exceed the needs of today’s laboratory. Bio SB has a wide array of nucleic acid extraction, detection, and quantification kits for interleukins, apoptosis, receptors, growth factors, oncogenes, genetic diseases, and microorganisms.

Kit Components 100 Reactions Storage
Pre-mixed Primers 1000 μl -20°C
Optimized PCR Buffer (Chemicals, enhancer, stabilizer, dNTPs) 750 μl x 4 tubes -20°C
Positive Control 100 μl -20°C
M.W. marker, 100 bp ladder 100 μl -20°C
ddH2O 1000 μl -20°C

-20°CMaxim’s RT-MPCR provides an accurate method to detect multiple gene expression by simultaneously amplifying all the genes under the same conditions. MPCR kits combine PCR amplification technology and multiple target detection throughput in a single tube. Maxim has developed proprietary technology that overcomes the non-specific amplification of PCR products between primers. The PCR products, also known as amplicons, are amplified efficiently to give relatively quantitative results from amplicon to amplicon, and sample to sample. MPCR’s capacity to simultaneously quantify multiple mRNA species from a single sample of RNA allows comparative analysis of different mRNA specieswithin samples. Variations in RNA isolation, initial quantification errors or tube-to-tube variations in RT and PCR can be compensated by including a known housekeeping gene. MPCR can also be performed on total RNA prepared by standard methods without further purification of poly (A) RNA.

Kit Components 50 Reactions 100 Reactions Storage
Optimized MPCR Buffer 1250 μL 1250 μL x 2 -20°C
Positive Control 50 μL 50 μL x 2 -20°C
Primer mixture 250 μL 250 μL x 2 -20°C
DNA M.W. Marker (100 bp Ladder) 100 μL 100 μL x 2 -20°C
ddH2O (DNase free) 2.0 mL 2.0 mL x 2 -20°C

Maxim’s RT-MPCR kits are ideal for use in the analysis and comparison of when, where, and to what degree genes are expressed, also known as gene expression profiling. Eventually, gene expression profiling will further our understanding of the control and mode of action of individual gene products. DNA chip technology is currently the most common method for determining gene abundance in a total RNA or poly(A) RNA sample, but this method is limited by low sensitivity and high cost. DNA chips may serve a better purpose as a discovery tool to identify genes related to a specific biological process, followed by using MPCR. MCPR can be a tool to further analyze the performance of genes in genetic profiling and quantative assays.

Major Advantages :

  • Diversity: MPCR Kits cover diverse applications from basic pathway gene expression profiling to diagnostic pathogen identification.
  • Conserves genetic material: only a single reaction is necessary to amplify multiple genes of interest.
  • Efficiency: One reaction is sufficient to amplify multiple genes simultaneously in a single reaction.

INTRODUCTION for our Real-Time PCR products can be found here

Real Time PCR Tests by Application

Real Time PCR for infectious, genetic & other diseases can be used to help professionals determine viral load, monitor disease progression and response to therapy. Major areas of application are bacterial/viral load quantification, pathogen detection & identification, differential gene expression, therapeutic drug monitoring, SNP genotyping, validation of RNAi, genetic disease analysis, food & environmental pathogen detection and forensic studies.

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Multiplex PCR Kits

Maxim’s complete Multiplex PCR Kits provide an accurate method to detect multiple gene expression by simultaneously amplifying all the genes under the same conditions. Multiplex PCR Kits combine PCR amplification technology and multiple target detection throughput in a single tube.

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Infectious Pathogen Gene Primers Kits

Maxim’s Infectious Pathogen Gene Primer-Single Primer Complete PCR Kits are designed to avoid formation of “primer-dimers” during amplification and provide all necessary reagents for 100 reactions. The PCR primers can be used for regular PCR or Real Time PCR. Maxim’s positive control primers are sequence-confirmed, copy number defined, and therefore can be used as a template for regular PCR or Real Time PCR.

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Human Gene Primer – Single PCR Kits

Maxim’s Human Gene Primer-Single Primer Complete PCR Kits are designed to avoid formation of “primer-dimers” during amplification and provide all necessary reagents for 100 reactions. The PCR primers can be used for regular PCR or Real Time PCR. Maxim’s positive control primers are sequence-confirmed, copy number defined, and therefore can be used as a template for regular PCR or Real Time PCR.

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DNA/RNA Ancillaries

DNA/RNA and PCR Ancillaries; buffers, gel loading buffer solutions, DNA M.W. markers, and other ancillaries of the highest quality for Molecular Biology applications.

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