TRAcP

Tartrate-resistant acid phosphatase (TRAcP) is a glycosylated monomeric metallo-enzyme expressed in mammals. It has a molecular weight of approximately 35 kDa, a basic isoelectric point (7.6 - 9.5), and optimal activity in acidic conditions. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. There are also certain pathological conditions whereby expression of TRAcP is increased. These include patients with Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher’s Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases.

Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts, which also express abundant TRAcP activity.

Anti-TRAcP is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

1. Janckila AJ, et al. Blood. 1995;May15;85(10):2839-44
2. Yaziji H, et al. Am J Clin Pathol. 1995;Oct;104(4):397-402
3. Janckila AJ, et al. J Histochem Cytochem. 1996;Mar;44(3):235-44
4. Janckila AJ, et al. Hybridoma. 1997;Apr;16(2):175-82
5. Hoyer JD, et al. Am J Clin Pathol. 1997;Sep;108(3):308-15
6. Janckila AJ, et al. Biotech Histochem. 1998;Nov;73(6):316-24

1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023) or EDTA (BSB 0030-BSB 0033).
5. Any of three heating methods may be used:
   • Electric Pressure Cooker
     Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
   • Water Bath Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes.
   • Conventional Steamer Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHC staining protocol.