HomeProductsDistributorsContact UsCareers

Back to Antibodies for Immunohistochemistry

PDF

Parvovirus B19

IHC of Parvovirus B19 on an FFPE Bone Marrow Infected Tissue

Description

Parvovirus B19 belongs to the Parvoviridae family of small DNA viruses. It is classified as Erythrovirus because of its capability to invade red blood cell precursors in the bone marrow.


Anti-Parvovirus antibody targets the capsid proteins VP1 and VP2 on Human Parvovirus. Parvovirus B19 infection has been implicated in spontaneous abortion in humans; thus, application of this antibody to placental tissues in such cases is appropriate. Parvovirus B19 is also associated with Erythema Infectiosum (Fifth Disease) in children and Acute Arthritis in adults, as well as Chronic Hemolytic Anemia, with some patients experiencing Aplastic crisis.

Antibody Type
Mouse Monoclonal
Clone
R92F6
Isotype
IgG1
Reactivity
Paraffin, Frozen
Localization
Nuclear, Cytoplasmic
Control
Infected Tissue
Storage
Store at 2°-8°C
Stability
2 years

For long-term storage of the concentrated antibody, it is recommended that aliquots of the antibody be frozen at -20°C in glycerol 50% (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Dilute using an antibody diluent such as ImmunoDetector Protein Block/Antibody Diluent (BSB 0040 and BSB 0041) or ImmunoDNA Background Blocker (BSB 0103-BSB 0107).

Presentation

Parvovirus is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Availability
Catalog No.
Antibody Type
Dilution
Volume/QTY
BSB 5854
Prediluted
Ready-To-Use
3.0 ml
BSB 5855
Prediluted
Ready-To-Use
7.0 ml
BSB 5856
Prediluted
Ready-To-Use
15.0 ml
BSB 5857
Concentrated
1:50-1:100
0.1 ml
BSB 5858
Concentrated
1:50-1:100
0.5 ml
BSB 5859
Concentrated
1:50-1:100
1.0 ml
BSB 5860
Control Slides
 
5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
References
  1. Loughrey AC, et al. J Med Vir. 1993;39:97-100
  2. Moore L, et al. Med J Australia. 1993;159:344-345
  3. Morey AL, et al. J Path. 1992;166:105-108
  4. O’Neill HJ, et al. 1992;123:125-134
  5. Silverberg SG, et al. 1997;219-220

Recommended Immunohistochemical Protocol

Pretreatment
  1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
  2. Air dry for 2 hours at 58° C.
  3. Deparaffinize, dehydrate and rehydrate tissues.
  4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023) or EDTA (BSB 0030-BSB 0033).
  5. Any of three heating methods may be used:
    • Electric Pressure Cooker
      Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
    • Water Bath Method
      Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes.
    • Conventional Steamer Method
      Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
  6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
  7. Wash slides with IHC wash buffer or DI water.
  8. Continue IHC staining protocol.

Immunohistochemical Protocol

Step

ImmunoDetector
(AP or HRP)

PolyDetector
(AP or HRP)
Peroxidase/AP Block
5 minutes
5 minutes
Primary Antibody
30 minutes
45 minutes
Secondary Biotinylated Link
10 minutes
Not Applicable
AP or HRP Label
10 minutes
45 minutes
Substrate-Chromogen
5-10 minutes
10 minutes
Counterstaining
Time varies with counterstain
Time varies with counterstain