Mammaglobin

Mammaglobin is a gene that encodes a 10 kDa glycoprotein. In humans, expression of the gene is limited to the adult mammary gland. A correlation between increased expression of the gene and Breast Cancer has been reported. Mammaglobin mRNA is present in high levels in human Breast Cancer cell lines and primary Breast Cancers. High levels of mRNA have been detected in normal human sweat glands as well, but are absent in Sweat Gland Tumors.

Anti-Mammaglobin (31A5) has been shown to be effective in detecting up to 85% of Breast Carcinomas using immunohistochemical techniques. Studies investigating the detection of mRNA by RT PCR from circulating carcinoma cells in the peripheral blood of Breast Cancer patients have shown that mammaglobin is a highly-specific marker and correlates with several prognostic factors, such as lymph node involvement.

Anti-Mammaglobin (31A5) is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

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4. Sjodin A, et al. J Invest Dermatol. 2003;Aug.v.121(2);428-429

1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023) or EDTA (BSB 0030-BSB 0033).
5. Any of three heating methods may be used:
   • Electric Pressure Cooker
     Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
   • Water Bath Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes.
   • Conventional Steamer Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHC staining protocol.