EGFR

Epidermal Growth Factor Receptor (EGFR) is the receptor for epidermal growth factor (EGF). It is a member of the ErbB family receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER-2 neu (ErbB-2), HER-3 (ErbB-3) and HER-4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Mutations involving EGFR could lead to its constant activation, which could result in uncontrolled cell division – a predisposition for cancer. Consequently, mutations of EGFR have been identified in several types of cancer, and EGFR is the target of an expanding class of anticancer therapies. EGFR is overexpressed in tumors of the breast, brain, bladder, lung, stomach, head, neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in Squamous Cell Carcinomas.

EGFR is a Mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

1. N/A

1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to enzymatic digestion with ImmunoDNA Digestor (BSB 0108 – BSB 0112) or similar product (Proteinase K) using the following protocol:
   • Pre-warm the ImmunoDNA Digestor solution to room temperature
   • Incubate tissue sections with the ImmunoDNA Digestor solution for 10 minutes at room temperature or for 5 minutes at 37° C.

1. Wash slides with IHC wash buffer or DI water.
2. Continue IHC staining protocol.