Cytomegalovirus

Cytomegalovirus (CMV) is a virus of the Herpes-virus group; in humans it is commonly known as HCMV or Human Herpesvirus 5 (HHV-5). CMV belongs to the Betaherpesvirinae subfamily of Herpesviridae, which also includes Roseolovirus. CMV especially attacks salivary glands. CMV infection can also be life-threatening for patients who are immunocompromised (e.g., patients with HIV or organ-transplant recipients). CMV viruses are found in many mammal species, but CMV species isolated from animals di er from human CMV in terms of genomic structure, and have not been reported to cause human disease.

This Anti-cytomegalovirus antibody cocktail reacts with two different epitopes. The DDG9 antibody reacts with a 76 kDa protein produced by CMV. CCH2 antibody reacts with the early DNA-binding protein p52. There is no cross-reactivity with other Herpesviruses or Adenoviruses. CMV infection is usually seen in immunocompromised patients and involves the GI tract, lung, heart and liver, as well as other organs.

CMV is a cocktail of two mouse monoclonal antibodies derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

1. Plachter B, et al. Virus Research. 1992;24:265-76
2. Silverberg SG, et al. Principles and Practice of Surgical Pathology and Cytopathology. 1997;217-218.

1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023) or EDTA (BSB 0030-BSB 0033).
5. Any of three heating methods may be used:
   • Electric Pressure Cooker
     Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
   • Water Bath Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes.
   • Conventional Steamer Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHC staining protocol.