CD5

CD5 is a glycoprotein monomer with an MW of 67,000 belonging to the scavenger receptor cysteine-rich (SRCR) family of extracellular domain-like structures, and it possesses a large cytoplasmic domain suitable for signal transduction.

CD5 is a T-cell marker that also reacts with a range of neoplastic B-cells, e.g., B-cell Chronic Lymphocytic Leukemia (B-CLL), B-cell Small Lymphocytic Lymphoma (B-SLL), and Mantle Cell Lymphoma. CD5 is expressed in T-lymphocyte subsets and is modulated during cellular activation; however, it does not react with granulocytes or monocytes.

CD5 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

1. Chan JKC, et al. Histopathology. 1994;25:517-536
2. Kasaian MT, et al. Proc of the Soc for Exp Bio and Med. 1991;197:226-241
3. Jones NH, et al. Nature. 1986;323:346-349

1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023) or EDTA (BSB 0030-BSB 0033).
5. Any of three heating methods may be used:
   • Electric Pressure Cooker
     Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
   • Water Bath Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes.
   • Conventional Steamer Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHC staining protocol.