CD138

CD138/Syndecan-1 is a transmembrane heparin-sulphate proteoglycan which is made up of one core protein and five glycosaminoglycans. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre B-cells and plasma cells but is absent from mature B-cells.

Anti-CD138/syndecan-1 is a useful marker for labeling normal and neoplastic plasma cells and Plasmacytoid Lymphomas. It is a selective marker for B-cell Lymphoblastic Leukemia and Lymphoplasmocytoid Leukemia. It is lost from the apoptotic myeloma cells, and thus, is a useful marker for viable Myeloma cells. Various forms of Hodgkin’s Disease have also shown positive staining with this antibody.

Anti-CD138/syndecan-1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

1. Chilosi M, Adami F, et al. Mod Pathol. 1999;Dec:12(12):1101-6
2. Sebestzen A, Berezi L, et al. Br J Haematol. 1999;Feb:104(2):412-9
3. Carbone A, Gaidano G, et al. Blood. 1998;Feb:1;91(3):747-55

1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023) or EDTA (BSB 0030-BSB 0033).
5. Any of three heating methods may be used:
   • Electric Pressure Cooker
     Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
   • Water Bath Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes.
   • Conventional Steamer Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHC staining protocol.