CD10

CD10, also known as neutral endopeptidase (NEP), Neprilysin, and common Acute Lymphoblastic Leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer’s Disease.

CD10 is a useful marker for the characterization of childhood Leukemia and B-cell Lymphomas. This antibody reacts with the antigens of Lymphoblastic, Burkitt’s, and Follicular Lymphomas, and Chronic Myelocytic Leukemia. Also, CD10 detects the antigen of glomerular epithelial cells and the brush border of the proximal tubules. This characteristic may be helpful in interpreting renal ontogenesis, in conjunction with other markers. Other non-lymphoid cells that are reactive with CD10 are breast myoepithelial cells, bile canaliculi, neutrophils, a small population of bone marrow cells, fetal small intestine epithelium, and normal fibroblasts.

CD10 is a mouse monoclonal antibody from supernatant diluted in Phosphate Buffered Saline, pH 7.6, with protein base, and preserved with Sodium Azide preservative.

1. Pardossi-Piquard R, et al. Journal of Neurochemistry. 2006;97(4):1052-6
2. Haralambidou S, at al. J Clin Pathol. 1987;40:490-493
3. Mechterscheimer, et al. Am J of Pathol. 1989;134(5):961-965

1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023) or EDTA (BSB 0030-BSB 0033).
5. Any of three heating methods may be used:
   • Electric Pressure Cooker
     Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
   • Water Bath Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes.
   • Conventional Steamer Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHC staining protocol.