CA-125

CA-125 reacts with malignant ovarian epithelial cells. CA-125 also reacts with antigens in Seminal Vesicle Carcinoma and Anaplastic Lymphoma.

In adult tissues, CA-125 is found in epithelial cells of Fallopian tube, endometrium and endocervix, pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland, and mammary gland. It is also found in mesothelial cell lining of pleura, pericardium and peritoneum. It is found in ovarian tumors of serous, endometrioid or clear-cell types and Adenocarcinomas of Mullerian type.

CA-125 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

1. O’Brien TJ, et al. Amer J Obstet Gynecol. 1991;165(6):1857
2. Hardardottir H, et al. Amer J Obstet Gynecol. 1990;163(6):1925
3. Dabawat S, et al. Int J Gyn Path. 1983;2:275-285
4. Davis H, et al. Cancer Res. 1986;46:6143-6148
5. Nouwen E, et al. Cancer Res. 1986;46:866-876

1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (BSB 0020-BSB 0023) or EDTA (BSB 0030-BSB 0033).
5. Any of three heating methods may be used:
   • Electric Pressure Cooker
     Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
   • Water Bath Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes.
   • Conventional Steamer Method
     Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHC staining protocol.