CISH-Fast-HPV

CISH of HPV on an FFPE Cervical Cancer

CISH-Fast-EBV

CISH of HPV on an FFPE Cervical Cancer

CISH-Fast-lg-Kappa

CISH of HPV on an FFPE Cervical Cancer

Achieving Chromogenic in situ Hybridization Results in Just 4 Hours!

Introduction

The ZytoFast® products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-κ and Ig-λ light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples. The Signal intensity of ZytoFast probes is increased even more when using the ZytoFast PLUS Implementation Kits

Method Description – ZytoFast

zytofast_method_smallThe ZytoFast system uses oligonucleotide probes tagged with Biotin (1) which are detected using enzyme-conjugated streptavidin targeting the tag (2).

The enzymatic reaction of chromogenic substrates (3), e.g. BCIP/NBT or AEC, leads to the formation of strong color precipitates that can be visualized by light microscopy.

Method Description – ZytoFast PLUS

zytofast_plus_method_small

The ZytoFast PLUS system uses Digoxigenin-labeled probes (1) which are detected using primary antibodies (2). These antibodies are detected by polymerized enzyme-conjugated secondary antibodies (3). The enzymatic reaction of chromogenic substrates (4), e.g. NBT/BCIP, AEC or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.

High Sensitivity and Specificty

All ZytoFast probes are tagged either with Biotin or Digoxigenin using the unique ZytoFast HighTag System providing improved signal intensity! High specificity without risk of cross-hybridizations is obtained due to optimized oligonucleotide probes.

Advantages of Fast-CISH

  • Simultaneous observation of tissue morphology and CISH signals
  • No risk of false positives due to mispriming or contamination as with PCR
  • Easy method comparable to IHC
  • No costly equipment needed
  • Ability to test archival specimens
  • High sensitivity and specificity

ZytoFast – Flexibility that meets your Needs

Several ZytoFast and ZytoFast PLUS CISH Implementation Kits including different enzyme/substrate combinations can be combined with any separately available Biotin-labeled ZytoFast probe and Digoxigenin-labeled ZytoFast probe, respectively, to meet your preferences concerning the detection chemistry, counterstaining, and embedding. Each ZytoFast and ZytoFast PLUS CISH Implementation Kit includes a detailed protocol, all necessary reagents as well as positive and negative control probes for versatile use in DNA as well as RNA in situ Hybridizations.

ZytoFast Complete Kits

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VOL/QTY
 T-1005-40 ZytoFast human lg-kappa/lg-lambda CISH Kit  40
 T-1105-40 ZytoFast human lg-kappa/lg-lambda Permanent CISH Kit  40

ZytoFast Biotin & Digoxigenin Labeled Probes

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ZytoFast Biotin Labeled Probes

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VOL/QTY
T-1013-400  ZytoFast CMV Probe  400 μl
 T-1014-400  ZytoFast EBV Probe  400 μl
 T-1015-400  ZytoFast human lg-kappa Probe  400 μl
 T-1016-400  ZytoFast human lg-lambda Probe  400 μl
 T-1019-100 ZytoFast RNA (-) Control Probe  100 μl
 T-1020-100  ZytoFast 28S RNA (+) Control Probe (Biotin labeled)  100 μl
 T-1022-100  ZytoFast DNA (+) Control Probe  100 μl
 T-1023-100  ZytoFast DNA (-) Control Probe  100 μl
T-1032-400  ZytoFast HPV type 6/11 Probe  400 μl
 T-1035-400  ZytoFast HPV type 16/18 Probe  400 μl
 T-1038-400  ZytoFast HPV type 31/33 Probe  400 μl

ZytoFast Digoxigenin Labeled Probes

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ZytoFast CISH Detection Systems for the Detection of Biotin Probes

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ZytoFast CISH Detection Systems for the Detection of Digoxigenin Probes

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