IHC of Treponema pallidum on an FFPE Infected Skin Tissue

United States
International
Research Use
Safety Data Sheet

SDS

Control Slides
Intended Use For Analyte Specific Reagent
Summary and Explanation

Treponema pallidum is a spirochaete bacterium. The treponemes have a cytoplasmic and an outer membrane. The shape of T. pallidum is flat and wavy, unlike the other spirochetes, which are helical. Using light microscopy, treponemes are only visible using dark field illumination. They are Gram negative, but some regard them too thin to be Gram stained. T. pallidum is a motile spirochaete that is generally acquired by close sexual contact, entering the host via breaches in squamous or columnar epithelium. The organism can also be transmitted to a fetus by transplacental passage during the later stages of pregnancy, giving rise to congenital syphilis. The helical structure of T. pallidum allows it to move in a corkscrew motion through a viscous medium such as mucus. It gains access to the host’s blood and lymph systems through tissue and mucous membranes.

Detection of Treponema pallidum can be difficult, and the correct diagnosis of secondary syphilis is critical. Diagnosis of syphilis is usually based on clinical presentation, dark-field microscope analysis, and serological tests. T. pallidum can be also evidenced by Immunohistochemistry in up to 90% of the samples with the bacteria located in the epidermis and the upper dermis of formalin-fixed paraffin-embedded tissues.

Antibody Type Rabbit Polyclonal Clone Polyclonal
Isotype IgG Reactivity Paraffin, Frozen
Localization Cell Wall Control Treponema pallidum infected tissue
Presentation

Treponema pallidumid a purified immunoglobulin fraction of rabbit antiserum that is filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3232 Prediluted Ready-To-Use 3.0 ml
BSB 3233 Prediluted Ready-To-Use 7.0 ml
BSB 3234 Prediluted Ready-To-Use 15.0 ml
BSB 3235 Concentrated 1:250-1:1000 0.1 ml
BSB 3236 Concentrated 1:250-1:1000 0.5 ml
BSB 3237 Concentrated 1:250-1:1000 1.0 ml
BSB 3238 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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