Overview & Features

The Bio SB TintoStainer is an automated 36, 48, or 72 slide system for use in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). The TintoStainer is perfect for laboratories that have a need increase work-flow efficiency through automation, yet want to maintain low operating costs. The TintoStainer is a rapid, flexible, open and affordable solution which allows for fast implementation of laboratory protocols while keeping a compact footprint. The TintoStainer features an integrated slide and reagent bar-coding system to ensure that the right reagents and slides are used with a selected protocol, while reducing incidences of user-error.

Systems from competing companies are not only expensive to purchase but are “closed”, limiting the detection systems and antibodies available for use. This leaves laboratories at the mercy of supplier price hikes and a limited catalog of reagents to choose from. The TintoStainer is an open system which allows for the implementation of any ancillary, antibody, detection system, or chromogen from any supplier.

To ensure successful integration into your laboratory, the TintoStainer comes bundled with Bio SB ancillaries, Tinto prediluted antibodies, and detection kits. Additionally, the TintoStainer comes with complete setup, support, repair, and training for 1 year so that laboratories feel confident that the TintoStainer is always up and running.

TintoStainer in the Lab

• Barcode Scanning System

The Bio SB TintoStainer barcode scanner scans both labelled slides and reagents. The barcode scanning system processes up to 36 slides in under 3 minutes, ensuring rapid protocol implementation.

• Precise Reagent Application

The TintoStainer can control how much reagent you want dispensed on every slide. Now laboratories can have complete control over how much reagent is used per slide and customize protocols according to individual needs.

• Reagent & Slide Barcode Labeling

Track reagents and quickly run experiments with the TintoStainer’s barcode labeling system. All labels are printed directly from the TintoStainer and are HIER and Xylene resistant. All reagent and slide barcodes are uniquely generated, reducing user error.

TintoStainer Probe & Slides

TintoStainer Equipment & Catalog Numbers


Automated Instruments TintoStainer Labels and Printers
BSB 7029 TintoStainer 36 (110V) BSB 7059 Reagent Barcode Label Kit
BSB 7032 TintoStainer 36 (240V) BSB 7060 PT Module Pump Kit
BSB 7051 TintoStainer 48 (110V) BSB 7061 Autostainer Slide Rack
BSB 7074 TintoStainer 48 (240V) BSB 7062 Label Printer with Cable, 220V
BSB 7052 TintoStainer 72 (110V) BSB 7075 Label Printer with Cable, 110V
BSB 7053 TintoStainer 72 (240V) TintoStainer Accessories
Epitope Retrieval BSB 7063 DAB-Away Kit
BSB 7030 TintoRetriever PT Module (110V) BSB 7064 PT Module Pump Kit
BSB 7033 TintoRetriever PT Module (240V) BSB 7065 Autostainer Slide Rack
BSB 7008 TintoRetriever Pressure Cooker BSB 7067 Waste Tubing
TintoStainer Vials and Reagents BSB 7068 Buffer In-line Tubing
BSB 7054 12ml Barcode Vials (100 vials) BSB 7070 Reagent Rack
BSB 7056 Reagent Tray for TintoStainer 48 BSB 7071 Buffer Bottle
BSB 7057 2nd Reagent Tray for TintoStainer 72 BSB 7072 Carboy for Waste/Buffer
BSB 7058 Tray for 40 Autostainer vials BSB 7073 Carboy Lid

 

TintoStainer Components

 

TintoStainer ComponentsTintoStainer

A. TintoStainer System B. Slide Barcode Printer C. Computer Unit

D. Reagent Barcode Printer E. 12 Slide Rack F. Reagent 12mL Vial

 

 


 

Key Concepts and Terminology

 

Reagent
Specific reagent as stored in the reagents lists or in a specific protocol. It generally includes a name, bar-code, lot number/expiration date and incubation time. Antibodies are a form of reagent that also include antigen retrieval information. TintoStainer Reagent


Antibody
A single reagent step that also includes antigen retrieval information. An antibody must be utilized in a protocol to be used on a slide. TintoStainer Antibody


Protocol
A protocol is composed of all the IHC steps that will take plance on a slide to complete a given stain. The details for each unique protocol are stores as a file called an Auto-program (*.aut file format) in the auto-stainer operating directory. The protocol is made up of unique reagents, incubation times and antigen retrieval. Example is shown below.

TintoStainer Protocol

 

Protocol Template
A protocol template is the number and arrangement of programmable stepts that protocols (antibody protocols and auto-programs) can be created in. Protocol templates are often configured in the protocol template screen. Protocols must match the default protocol template in order to be used.
TintoStainer Protocol Template
Default Template TintoStainer Protocol Template
The primary protocol template. Protocols must match the default template in order to be used (labels printed and run).
Optimization
Determining the correct working protocol for a given antibody. This does not include validation.
Validation
Ensuring an optimized protocol meets desired results via laboratory defined testing. See the assay Validation Reccomendations section in the manual for more information.
Detection System Protocol
This is a protocol that has been saved with the antibody column left blank. Detection System protocols can be assigned to specific antibody reagents in the antibody edit lists screens to create a full protocol.

Customization of Slide & Reagent Labels

Slide Label Printing

Reagent Label Printing

1. Data Customized in “Design Slide Label” in initialize screen. 1. Data customized in “design reagent label” in initialize screen…
TintoStainer Slide Label Printing TS1
2. Is retrieved from data input by user on “Slide Information” menu on programming grid. 2. Is retrieved from “Edit reagents list” menu on programming grid.
TintoStainer TS2
3. Which is incorporated onto slide printout. 3. Which is incorporated onto reagent printout.
Slide Printout TS3

 

Reagent Drop Zone

The TintoStainer also allows a user to select a specific drop zone for reagent. This feature allows a user to ensure that reagent is applied specifically to tissue sections that have a specimen.

1. To pick a reagent drop zone, simply click on the “Dispense” column in the protocol template.
2. Make sure that the proper “Drop Zone” is applied to the tissue, depending on the part of the slide your tissue is located. Default drop zone and volume can be changed in initialize screen.
3. The area highlighted in yellow is where reagent will be dispersed.
4. To pick a reagent drop zone, simply click on the “Dispense” column in the protocol template.
5. The “uL” symbol corresponds to the top of the slide.
6. More than one “drop zone” can be picked, although more reagent will be used.
7. The area highlighted in yellow is where reagent will be dispersed.

Programming a Custom Protocol

The TintoStainer comes programmed with protocols for the Bio SB ImmunoDetector HRP/AP, Bio SB PolyDetector HRP/AP, and Bio SB PolyDetector PLUS HRP Detection Systems. However, some laboratories may need to build a protocol template customized to their needs. The TintoStainer has an easy to use methodology.

1. On the programming grid screen, press “Protocol Template” button in bottom left hand corner. Custom Protocol Programming
2. Left side of screen will list steps that you can add to your protocol. Select the steps you wish to incorporate, including any necessary washes.
3. Before the chromogen option is incorporated into your template, ensure that you select the *Switch step. This step ensures waste generated from chromogen goes into the hazardous waste container.
4. When done building protocol, select “New Template” button.
5. Select “save to disk now” option.
6. Type in Protocol name
7. Select “Yes”.

 

• Note: All protocols below have Immunowasher Rinses between each step unless otherwise indicated.
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