Overviews and Features
The Bio SB TintoDetector is a compact molecular pathology system which allows for full implementation of immunohistochemical (IHC), immunocytochemical (ICC), chromogenic in-situ hybridization (CISH) and fluorescent in-situ hybridization (FISH) protocols in your diagnostic laboratory. The Bio SB TintoDetector is intended for laboratories which require flexibility in their diagnostic needs, yet may not require high volume slide output offered by automated systems, such as the Bio SB
TintoStainer. The Bio SB TintoDetector is an open system that allows for the use of any detection system or reagent. The TintoDetector paired with accessories like the Bio SB TintoRetriever pressure cooker and Bio SB TintoDetector slide holder allows laboratories to implement a wide variety of lab protocols at a low cost.
- Eight staining dishes for reagents and buffers.
- Built in incubator for ISH protocols that reaches 110 degrees celsius, stores 3 incubator presets.
- Stainless steel construction that is chromogen and xylene resistant.
- Small bench footprint with low power requirements.
- Supports ICC, IHC, CISH, and FISH protocols. Open system
TintoDetector Lab Applications
The TintoDetector Incubator is capable of reaching temperatures up to 110 degrees celsius, and is capable of storing 3 temperature presets. The incubator is used to apply varying temperatures typicaly used inCISH and FISH protocols that involve the denaturing of nucleic acids, probe hybridization and other steps related to CISH & FISH implementation.
The TintoDetector Slide Holder is an extremly durable capillary gap slide holder that is capable of holding 20 capillary gap slides, and easily fits into the TintoDetector Incubator. The TintoDetector slide holder can be used for all IHC, ICC, IF, CISH and FISH applications.
When using the TintoDetector slide Holder, be sure the following procedures are followed:
- Slides are paired face to face.
- if a single slide or odd number must be used, pair with a blank slide.
- Insert slides so that portion of slide with white triangles faces downward when unit is held.
- Unsure that Prone On Plus slides are used.
- Ensure all bottom edges of slides are aligned to ensure proper capillary gap action.
The TintoDetector 30 well reagent holder allows for the application of up to 200 microliters of reagent to a paired set of slides. The 30-well reagent holders can be used to apply any reagent used in IHC, ICC, IF, CISH and FISH Protocols.
|BSB 7000||TintoDetector System|
|BSB 7002||TintoDetector Incubator|
|BSB 7004||TintoDetector 30-Well Reagent Holder|
|BSB 7036||TintoDetector Absorbent Pads|
TintoDetector Wash Procedure
Step 1 – Load Slides
Load slides in TintoDetector Holder, face to face and properly ordered.
Step 2 – Apply Reagents to Reagent Holder
Apply reagent to TintoDetector 30-well Reagent Holder. Each reagent well can hold about 200 μl of reagent.
Step 3 – Draw Reagents into Slides
Place TintoDetector slide holder over 30-well reagent holder, ensuring that reagents line up with slides. Press slide holder gently against reagent holder. Capillary gap action will draw reagent. Transfer TintoDetector Slide Holder to Incubator.
Step 4 – Incubate
Incubate Slide Holder using the TintoDetector Incubator.
Step 5 – Rinse
After the reagent incubation, eliminate the used reagent into an absorbent pad by gently pressing the slides on the absorbent pad to drain the liquid. Draw washing buffer into the capillary space and repeat the washing process 3 to 5 times. After washing proceed to draw the next step reagent before another incubation.