IHC of TdT on an FFPE Thymus Tissue

Specification Sheets
Safety Data Sheet


Intended Use For In Vitro Diagnostic Use
Summary and Explanation Terminal Deoxynucleotidyl Transferase (also known as TdT and terminal transferase) is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells and acute Lymphoblastic Leukemia/ Lymphoma cells. TdT catalyzes the addition of nucleotides to the 3’ terminus of a DNA molecule. Unlike most DNA polymerases, it does not require a template. The preferred substrate of this enzyme is a protruding 3’ overhang, but it can also add nucleotides to blunt or recessed 3’ ends.TdT is normally found in cortical thymocytes and primitive lymphocytes. TdT antibody detects its antigen found in the nucleus of normal hematopoietic cells, normal cortical thymocytes and in the cytoplasm of megakaryocytes of the bone marrow. TdT expression is seen in over 90% of Acute Lymphocytic Leukemia cases with the exception of pre-B-Cell ALL, and normal mature T- or B-lymphocytes. TdT is positive for approximately one third of all cases of Chronic Myeloid Leukemia, making it a good indicator of better response to chemotherapy.
Antibody Type Rabbit Monoclonal Clone RBT-TdT
Isotype IgG Reactivity Paraffin, Frozen
Localization Nuclear Control TdT Positive Lymphoma, Thymus
Presentation TdT is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2265 Prediluted Ready-To-Use 3.0 ml
BSB 2266 Prediluted Ready-To-Use 7.0 ml
BSB 2267 Prediluted Ready-To-Use 15.0 ml
BSB 2268 Concentrated 1:50-1:200 0.1 ml
BSB 2269 Concentrated 1:50-1:200 0.5 ml
BSB 2270 Concentrated 1:50-1:200 1.0 ml
BSB 2271 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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