IHC of Smoothelin on an FFPE Uterus Tissue

IHC of Smoothelin on an FFPE Uterus Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Smoothelin is a constituent of the smooth muscle cell cytoskeleton protein exclusively found in differentiated smooth muscle cells (SMC). Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. To distinguish bladder muscularis mucosae (MM) from muscularis propria (MP) muscle bundles is crucial for accurate staging of bladder carcinoma.
Strong smoothelin expression is nearly exclusively observed in muscularis propria. Therefore, the staining pattern of MP (strongly positive) and MM (negative or weakly positive) makes this technique an attractive diagnostic tool for the sometimes difficult task of staging bladder urothelial carcinoma such as in transurethral resection specimens of urinary bladder tumrors. Anti-Smoothelin can also be useful in differentiationg between benign (+) and malignant smooth muscle tumors (-).
Antibody Type Mouse Monoclonal Clone R4A
Isotype IgG1 Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Leiomyoma, Bladder. Prostate, Myometrium, Fallopian Tube, Colon, Transitional Cell Carcinoma
Presentation Smoothelin is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2189 Prediluted Ready-To-Use 3.0 ml
BSB 2190 Prediluted Ready-To-Use 7.0 ml
BSB 2191 Prediluted Ready-To-Use 15.0 ml
BSB 2192 Concentrated 1:25-1:100 0.1 ml
BSB 2193 Concentrated 1:25-1:100 0.5 ml
BSB 2194 Concentrated 1:25-1:100 1.0 ml
BSB 2195 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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