IHC of PU.1 on an FFPE Lymphoma Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||PU.1 is a member of the Ets family of transcription factors and is required for the development of multiple hematopoietic lineages. It plays a pivotal role in normal myeloid differentiation and regulates the expression of immunoglobulin and other genes that are important for B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B lymphocytes, with the exception of plasma cells.
PU.1 is expressed in germinal center B-cells and mantle B-cells. The antibody is positive in various lymphomas including B-Chronic Lymphocytic Leukemia, Mantle Cell Lymphoma, Follicular Lymphoma, Marginal Zone Lymphoma, Burkitt Lymphoma, Diffuse Large Cell Lymphoma, Diffuse Large B-cell Lymphoma, T-cell rich B-cell Lymphoma, Nodular Lymphocyte Predominant Hodgkin Lymphoma. It has been demonstrated that a high level of expression of GC antigens (including PU.1) has a positive association with longer overall survival and progression free survival in the case of Follicular Lymphoma.
|Antibody Type||Rabbit Monoclonal||Clone||EP18|
|Localization||Nuclear||Control||Tonsil, Lymph Node|
|Presentation||PU.1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|