IHC of PTEN on an FFPE Prostate Carcinoma Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use.
Summary and ExplanationPhosphatase and tensin homolog (PTEN) is a protein that, in humans, is encoded by the PTEN gene. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating Akt/PKB signaling pathway.PTEN is one of the most commonly lost tumor suppressors in human cancer; in fact, up to 70% of men with prostate cancer are estimated to have lost a copy of the PTEN gene at the time of diagnosis. During tumor development, mutations and deletions of PTEN occur that inactivate its enzymatic activity leading to increased cell proliferation and reduced cell death. Frequent genetic inactivation of PTEN occurs in glioblastoma, endometrial cancer, and prostate cancer; and reduced expression is found in many other tumor types such as lung and breast cancer. In breast and prostate cancer, loss of PTEN expression has been shown to correlate positively with advanced stage. Furthermore, PTEN mutation also causes a variety of inherited predispositions to cancer.
Antibody TypeRabbitt MonoclonalCloneRBT-PTEN
IsotypeIgGReactivityParaffin, Frozen
LocalizationCytoplasmic, NuclearControlColon, Thymus, Skin, Kidney, Breast, Prostate, Breast Carcinoma, Prostatic Carcinoma
PresentationPTEN is a rabbit monoclonal antibody derived from cell culture supernatant that
is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing
BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 2643PredilutedReady-To-Use3.0 ml
BSB 2644PredilutedReady-To-Use7.0 ml
BSB 2645PredilutedReady-To-Use15.0 ml
BSB 2646Concentrated1:25-1:1000.1 ml
BSB 2647Concentrated1:25-1:1000.5 ml
BSB 2648Concentrated1:25-1:1001.0 ml
BSB 2649Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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