IHC of B7/H3 on FFPE Colon Metastasis to Lung Tissue (HRP Blue)
IHC of SATB2 on FFPE Colon Carcinoma Tissue (HRP Blue)
IHC of NKX3.1 on FFPE Prostatic Adenocarcinoma Tissue (HRP Blue)
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||The PolyDetector HRP Blue Substrate-Chromogen Kit is suitable for use with peroxidase detection systems, which allow the demonstration of cell antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations. The substrate-chromogen is the final step in the detection portion; it enables the antibody-antigen or nucleic acid complex to be viewed under a light microscope. This occurs because HRP Blue acts as an electron donor in the presence of the enzyme horseradish peroxidase; HRP Blue gets oxidized and produces a vibrant blue color at the site of the target antigen or nucleic acid. This substrate-chromogen is particularly useful in tissues where there is endogenous melanin or with multiplex IHC or CISH.
HRP Blue is suitable for aqueous mounting. HRP Blue is soluble in organic solvents like alcohol or xylenes and therefore cannot be mounted with alcohol/xylene protocol unless using the Bio SB ChromoProtector (BSB 0151 – BSB 0156), then an organic Permanent Mounting medium such as the PermaMounter (BSB 0094-0097) or similar permanent mounting media, or the solvent-free XyGreen PermaMounter (BSB 0169-0174).
|Presentation||The PolyDetector HRP Blue Substrate-Chromogen Kit contains a liquid HRP Blue Chromogen dissolved in a polymer solution and an HRP Blue Buffer Substrate containing a buffer, hydrogen peroxide as a substrate, and a non-azide anti-microbial.|