IHC of PD-1 / CD279 on an FFPE Tonsil Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

Programmed Death 1 (PD-1 or CD279) is a Type I membrane protein comprised of 268 amino acids. PD-1 is a member of the extended CD28/CTLA-4 family of T-cell regulators. PD-1 is expressed on the surface of activated T-cells, B-cells, and macrophages. In comparison to CTLA-4, PD-1 more broadly negatively regulates immune responses.

New data suggests that expression of PD-L1 on tumor cells inhibits anti-tumor activity through engagement of PD-1 on effector T-cells. Expression of PD-L1 on tumors is correlated with reduced survival in esophageal, pancreatic and other types of cancers, highlighting the relevance of exploring the PD-1 pathway as a target

for immunotherapy. Studies have found that PD-1 is expressed on most T-cells and a small subset of B-cells in the light zone of germinal centers, but not elsewhere in the tonsil. On that basis, it was postulated that PD-1 may play a role in the process of clonal selection of centrocytes, which occurs in this subanatomic site in germinal centers. PD-1 is a new marker of Angioimmunoblastic Lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and bcl-6, PD-1 is expressed by few B-cells, so it may be a more specific and useful diagnostic marker in Angioimmunoblastic Lymphoma. It also seems to stain a greater percentage of CD3-positive neoplastic cells in Angioimmunoblastic Lymphoma than either CD10 or bcl-6.

Antibody Type Rabbit Monoclonal Clone EP239
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Membranous Control Tonsil, Lymph Node, Thymus, Spleen
Presentation PD-1 / CD279 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3148 Prediluted Ready-To-Use 3.0 ml
BSB 3149 Prediluted Ready-To-Use 7.0 ml
BSB 3150 Prediluted Ready-To-Use 15.0 ml
BSB 3151 Concentrated 1:50-1:200 0.1 ml
BSB 3152 Concentrated 1:50-1:200 0.5 ml
BSB 3153 Concentrated 1:50-1:200 1.0 ml
BSB 3154 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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